脱氧雪腐镰刀菌烯醇对人外周血单个核细胞HLA-I分子表达的影响  

The Effects of DON on HLA-I Expression of Human Peripheral Blood Mononuclear Cells in vitro

作　　者：李月红[1] 张祥宏[1] 邢凌霄[1] 严霞[1] 王俊灵[1] 王凤荣[1] 

机构地区：[1]河北医科大学基础所实验病理室,石家庄050017

出　　处：《细胞生物学杂志》2004年第3期297-300,共4页Chinese Journal of Cell Biology

基　　金：国家科技部基础研究重大项目前期研究专项资助项目(批准号:2001CCC00500);河北省自然科学基金(批准号:301350)

摘　　要：研究探讨了脱氧雪腐镰刀菌烯醇(DON)对人外周血单个核细胞HLA-I(human leuco-cyte antigen I)分子表达影响。采用流式细胞术(FCM)和免疫印迹方法研究了不同剂量DON对体外培养人外周血单个核细胞表面HLA-I分子表达的影响及其量效关系。FCM定量检测结果表明,不同浓度DON处理均可一定程度降低人外周血单个核细胞表面HLA-I分子的表达,DON 50ng/mL、100 ng/mL、1000 ng/mL和2000 ng/mL组HLA-I类分子的平均表达量分别为6.92±0.68、6.64±0.69、5.95±0.48和5.48±0.77,在50～2000ng/mL范围内随着DON浓度增加,外周血单个核细胞HLA-I分子表达降低,两者是显著负相关(r=0.737,P<0.01)。Western印迹结果显示,大剂量DON(1000ng/mL和2000 ng/mL)组人外周血单个核细胞HLA-I分子表达明显减弱。研究结果表明脱氧雪腐镰刀菌烯醇可剂量依赖地抑制体外培养的人外周血单个核细胞HLA-I分子的表达。To explore the effects of deoxynivalenol on HLA-Ⅰ expression of human peripheral blood mononuclear cells. Effects of deoxynivalenol at different concentration on HLA-Ⅰ expression of human peripheral blood mononuclear cells in vitro were studied with flow cytometry(FCM) analysis and Western blotting. FCM analysis indicated that HLA-Ⅰ expressions of human peripheral blood mononuclear cells were reduced in DON treatment groups (50ng/mL, 100ng/mL, 1000ng/mL and 2000ng/mL), especially in relatively higher concentration DON (1000ng/mL and 2000ng/mL) groups (P<0.01), compared to that in control. In the concentration range from 50ng/mL to 2000ng/mL, a significant concentration-depended negative correlation could be found between HLA- Ⅰ expression and DON concentration. Western blotting also confirmed that DON at higher concentration (1000ng/ mL and 2000ng/mL) could significantly inhibit HLA-Ⅰ expression at protein level. The results suggest that DON could reduce HLA-Ⅰ expression of human peripheral blood mononuclear cells in concentration-dependent manner in vitro.

关 键 词：脱氧雪腐镰刀菌烯醇 人 外周血单个核细胞 流式细胞光度术 WESTERN印迹 

分 类 号：Q26[生物学—细胞生物学]