源于129S1品系的小鼠胚胎干细胞系的建立及其生物学特性分析  

Establishment and Biological Characteristic Analysis of a New ES Cell Line Derived from 129S1 Mouse Strain

作　　者：杨桦 孙霞 徐国江 刘伟 王龙 赵旭东 陆荣华 费俭 王铸钢 

机构地区：[1]上海南方模式生物研究中心,上海201203

出　　处：《细胞生物学杂志》2004年第3期285-289,共5页Chinese Journal of Cell Biology

基　　金：国家高技术研究发展计划(863计划)项目(2001AA216081);上海市科技发展基金(STC-1-001);国家杰出青年科学基金(39925023)

摘　　要：从129S1小鼠早期胚胎的内细胞团分离、培养类胚胎样细胞,经反复传代,成功地建立了129S1小鼠胚胎干细胞系,命名为NM-2细胞系。形态学鉴定具有胚胎干细胞的典型形态特征,正常核型率为80％;呈碱性磷酸酶阳性、表达胚胎干细胞特异性转录因子OCT-4;体内分化后可形成源于三胚层的组织结构;经囊胚腔显微注射后所获得的子代个体中79％具有毛色嵌合表型;雄性嵌合个体中31％发生生殖腺嵌合;同时,通过育种观察到所有生殖腺嵌合体的子代小鼠表型正常。以上结果证实NM-2细胞系为一株具高生殖腺嵌合能力的小鼠胚胎干细胞系。A new ES cell line was isolated and cultured from inner cell mass collected from 129S1 mouse embryos at earfy stage, named as NM-2. The biological characteristics of this cell line were observed. It shows the typical morphology of ES cell clone, express embryo stem cell specific markers: alkaline phosphatase and tran- scription factor OCT-4. The normal karyotype rate was 80%. NM-2 could differentiate into various tissues from all three primary germ layers in vivo. Following injection into blastocysts, 79% of newborn mice were chimeras and 31% of male chimera showed germ-line transmission. All mice derived from germ-line chimeras show normal phenotype. The results demonstrated that NM-2 cell line was a mouse ES cell line with high potential for germ-line contribution.

关 键 词：胚胎干细胞 生殖腺嵌合 ES细胞系 129S1品系 小鼠 形态学 

分 类 号：Q813[生物学—生物工程]