串联质谱在多肽测序中的应用  

Application of Tandem Mass pectrometryon Peptide Sequence

作　　者：刘清萍[1] 刘中华[1] 唐新科[1] 陈平[1] 梁宋平[1] 

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081

出　　处：《生命科学研究》2004年第2期112-116,共5页Life Science Research

基　　金：国家自然科学基金资助重大项目(39990600)

摘　　要：采用纳升喷雾(Nano)技术和碰撞诱导解离(CIDcollisioninducddissociation)方法,在电喷雾-四极杆-飞行时间质谱(ESI-Q-TOFelectrspectrometryionization-quadrupole-timeofflight)上,对两种序列部分未知的天然多肽进行从头测序(denovosequence),结果证明质谱的denovosequence可以方便有效的解决传统的Edman降解法测序中常见的实际问题,如末位残基的丢失,赖氨酸和亮氨酸难鉴定等,此方法的建立是对Edman降解测序法很好的补充.Two natural peptides whose sequences are partly unknown through Edman Degration were analyzed by de novo sequence method of nano ESI MSMS.Results indicated that this method can effectively solve the difficulty occurring in Edman Degration,such as losing of the last residue, unidentification between leucine and lysine residues.The established method is a good supplement for Edman Degration.

关 键 词：电喷雾-四极杆-飞行时间质谱 从头测序 Edman降解 

分 类 号：Q503[生物学—生物化学] Q516