牛磺酸对心肌细胞凋亡的保护及其机制  

EFFECTS OF TAURINE ON THE APOPTOSIS OF CARDIAC MYOCYTES AND ITS MECHANISM

作　　者：李华[1] 万福生[1] 杨薇[1] 罗达亚[1] 

机构地区：[1]江西医学院生化与分子生物学教研室,江西南昌330006

出　　处：《南昌大学学报（理科版）》2004年第2期170-173,186,共5页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(30060029)

摘　　要：探讨牛磺酸对血管紧张素II(AngⅡ)诱导心肌细胞凋亡的保护作用及其机制。将培养Wistar乳鼠心肌细胞随机分成对照组、细胞凋亡组(10-5mol/LAngⅡ诱导)和不同浓度牛磺酸(10、20、40mmol/L)保护组,培养24h后观察各组心肌细胞存活率,细胞超微结构变化,末端标记(TUNEL)法和流式细胞仪检测细胞凋亡,同位素标记磷酸化底物法测定丝裂素活化蛋白激酶(MAPKs)和蛋白激酶C(PKC)活性。凋亡组心肌细胞凋亡率为21.23%,呈现凋亡形态结构特征,MAPK和PKC活性分别较对照组升高33.8%和81.8%。3个牛磺酸保护组凋亡率分别为4.89%、2.53%和1.58%,MAPK活性较凋亡组分别下降16.0%、23.5%、29.8%,PKC活性较凋亡组分别降低18.8%、29.8%、44.6%。牛磺酸能浓度依赖地抑制血管紧张素II诱导的心肌细胞凋亡,其机制可能与心肌细胞内MAPKs和PKC活性降低有关。To investigate the effects of taurine (Tau) on apoptosis in cultured cardiac myocytes of neonatal rat (NRCs) induced by angiotensinⅡ(AngⅡ) and possible mechanism.Cultured NRCs were randomly divided into the normal control ,apoptosis group(induced by 10-5 mol/L AngⅡ) and Tau treated groups (add 10-5 mol/L AngⅡand 10 or20 or 40mmol/L Tau) , incubated for 24 h, apoptosis of the cells was detected by transmission electron microscope, TUNEL and flow cytometry method, the activities of MAPK and PKC in the myocardial cell were determined by γ-32P-ATP phosphorylation substrate respectively.Apoptosis rate of cardiac myocytes is 21.3% in the apoptosis group, activities of MAPK and PKC increase significantly by 33.8% and (81.8%) respectively, compared with the control. Taurine increased significantly the survival rate of NRCs and attenuated apoptosis of NRCs induced by 10-5 mol/L of AngⅡ,compared with the apoptosis group, decrease activity of MAPK by 16.0%,23.5% and 29.8% in the Tau treated groups respectively and decreased activities of PKC in the cardiac myocytes by 18.8%,29.8% and 44.6% respectively. Conclusion: Taurine may inhibit the apoptosis of neonatal rat cardiac myocytes induced with AngⅡ, which is in dose-dependent manner, the mechanism may have relationship with decrease activities of MAPK and PKC in the cardiac myocytes of apoptosis.

关 键 词：凋亡 牛磺酸 心肌细胞 丝裂素活化蛋白激酶 蛋白激酶C 血管紧张素Ⅱ 

分 类 号：Q25[生物学—细胞生物学]