凝胶过滤层析对针刺全血清成分的分段分离研究  

The Study on Serum Segments Sepatated from the Acupuncture Serum by Gel Filtration

作　　者：徐慧明[1] 马淑兰[2] 杨永清[3] 崔龙萍[3] 王宇[3] 

机构地区：[1]上海市静安区中心医院,上海200040 [2]复旦大学上海医学院,上海200032 [3]上海市针灸经络研究所

出　　处：《上海针灸杂志》2004年第5期34-36,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金 (3 0 171177);上海市科委资助(0 2 DZ1915 0 -0 5 )

摘　　要：目的　为进一步分离、鉴定针刺血清中存在的有效成分 ,对针刺全血清进行预初分段分离。方法　采用凝胶过滤层析技术对针刺全血清进行分段分离 ,并将分离的血清经冷冻干燥制成干粉。结果　将针刺全血清分成 >4 5 k D、14 .3k D- 4 5 k D和 <14 .3k D 3个分子量段。结论　有利于进一步确定针刺血清中抗哮喘有效成分的大体所在 。Purpose To separate the acupuncture serum into three segments according to molecular weights.Method The acupuncture serum were separated by gel filtration with Sephadex G 75 and molecular standards.Results The acupuncture serum were separated into three segments according to molecular weight: >45 kD, 14.3 kD 45 kD and <14.3 kD.Conclusion It is helpful to confirm where do the active factors exist in the acupuncture serum and provide a foundation to the further study on the active factors.

关 键 词：凝胶过滤层析 针刺疗法 血清成分 哮喘 大鼠 活性因子 

分 类 号：R246.1[医药卫生—针灸推拿学] R256.12[医药卫生—中医临床基础]