人胃癌组织DNA甲基化酶、去甲基化酶与肿瘤相关基因的表达  

The expression of DNA methyltransferase,demethylase and tumor-associated genes in human gastric cancer

作　　者：程中华[1] 房静远[1] 杨丽[1] 陈萦晅[1] 陆嵘[1] 陆娟[1] 朱红音[1] 顾伟齐[1] 

机构地区：[1]上海第二医科大学附属仁济医院上海市消化疾病研究所,200001

出　　处：《中华消化杂志》2004年第4期203-206,共4页Chinese Journal of Digestion

基　　金：国家自然科学基金资助项目 (3 0 170 413 )

摘　　要：目的　探讨胃癌组织中甲基化酶、去甲基化酶基因与肿瘤相关癌基因和抑癌基因的关系。方法　取 2 8例胃癌手术标本的癌区、癌旁、正常组织 ,分别以RT PCR法和定量RT PCR法检测DNA甲基化酶 1(DNMT1)、去甲基化酶mbd2、甲基化结合蛋白MeCP2和 p16 INK4A、c myc等基因的转录水平 ,以相关分析等统计学处理研究各基因表达之间及分别与病理组织学之间的关系。结果　癌组织平均DNMT1和mbd2mRNA分别明显高于和低于正常组织。胃癌组织中c myc的表达增强 ,而MeCP2和 p16 INK4A无明显变化。在正常组织中 ,MeCP2与mbd2 ,p16 INK4A与MeCP2、mbd2 ,c myc与MeCP2的转录水平表现出一定的相关性 ,而当肿瘤发生后仅发现c myc与mbd2的表达呈负相关。以上各基因与肿瘤生物学行为均无相关性。结论　肿瘤相关基因mRNA的表达与甲基化酶DNMT1无关 ,而与甲基结合蛋白有关。Objective To investigate the relationship of the expression of DNA methyltransferase,demethylase(mbd2) and tumor-associated genes in human gastric cancer. Methods Tissue samples of cancerous,para-cancerous and normal gastric mucosa were obtained surgically from 28 primary gastric cancer patients. The transcription level of DNA methyltransferase 1 (DNMT1),mbd2,methyl-CpG binding protein (MeCP2),p16 INK4A and c-myc were determined by using real-time RT-PCR and RT-PCR. The relationship between the expression of DNA methylation-associated genes and tumor-associated genes was analyzed. Results The mRNA level of DNMT1 was higher and the mRNA level of mbd2 gene was lower in cancerous tissue than that in normal tissue. The expression of c-myc instead of p16 INK4A and MeCP2 was increased in cancer tissues. The mRNA level of c-myc related negatively to mbd2 when gastric cancer developed. However,there was no any close relation between the transcription level of all above genes and tumor biological behavior in human gastric cancer. Conclusion This study indicates that MeCP2 but not DNMT1 may contribute to the regulation of tumor-associated genes expression in human gastric cancer.

关 键 词：胃癌组织 DNA甲基化酶 去甲基化酶 肿瘤 基因表达 RT-PCR法 

分 类 号：R735.2[医药卫生—肿瘤]