黄芩苷对牙龈成纤维细胞和牙周膜细胞pro-MMP-1和MMP-3表达的影响  

Effects of baicalin on the expression of pro-MMP-1 and MMP-3 in human gingival fibroblasts and periodontal ligament cells

作　　者：李成章 [1] 曹正国 [1] 杨茹 [1] 尚姝环 [1] 金力坚 [2] Cobert EF  

机构地区：[1]430079,武汉大学口腔医学院牙周科 [2]香港大学牙科学院牙周科香港大学牙科学院牙周科

出　　处：《中华口腔医学杂志》2004年第3期197-200,共4页Chinese Journal of Stomatology

基　　金：湖北省科委基金资助项目 (2 0 0 1AA3 0 8B0 7)

摘　　要：目的　检测黄芩苷对白细胞介素 1β(IL 1β)诱导作用下人牙龈成纤维细胞 (HGF)分泌基质金属蛋白酶 1酶原 (pro MMP 1)的量和牙周膜细胞 (PDLCs)基质金属蛋白酶 3(MMP 3)表达的变化。方法　体外培养HGF和PDLCs,分别运用ELISA和免疫组化方法检测 pro MMP 1的量和MMP 3的表达。结果　与对照组的 (1 96 0± 0 176 ) μg/L相比 ,1μg/L的IL 1β能够显著促进HGF分泌 pro MMP 1(3 333± 0 12 3) μg/L ,且增加PDLCsMMP 3的表达 (P <0 0 0 1) ;加入黄芩苷后能降低HGF的pro MMP 1分泌量 ,其作用呈浓度 (10～ 10 0 0 μg/L)依赖性 ;黄芩苷对IL 1β诱导下PDLCs合成MMP 3的能力没有影响 ,但是能够抑制MMP 3的释放。结论　黄芩苷能够抑制由IL 1β介导的HGF分泌pro MMP 1和PDLCMMP 3的表达 ,提示黄芩苷可用于牙周病的防治。Objective To investigate the influence of baicalin on the IL-1β induced pro-MMP-1 in HGF and the effects of baicalin on MMP-3 expression in periodontal ligament cells(PDLCs). Methods The amount of secreted pro-MMP-1 and MMP-3 expression was detected by ELISA and cell immunochemistry. Results ①The amount of secreted pro-MMP-1(3.333±0.123)μg/L increased significantly following 1 μg/L of IL-1β,compared with control group(1.960±0.180 )μg/L. Addition of baicalin to cell culture medium for 1 hour following IL-1β decreased pro-MMP-1 secretion in a dose-dependent manner in the range of 10～1 000 μg/L. ②1 μg/L IL-1β could significantly stimulate the synthesis and secretion of MMP-3 in PDLCs. ③The baicalin could not interfere the synthesis of MMP-3,but could inhibit the release of MMP-3 from PDLCs. Conclusions Baicalin could inhibit the secretion of pro-MMP-1 and MMP-3 expression in IL-1β induced HGF and PDLCs,which suggests that baicalin may play an important role in preventing and treating periodontal disease.

关 键 词：黄芩苷 牙龈成纤维细胞 牙周膜细胞 MMP-3 基质金属蛋白酶3 基质金属蛋白酶1酶原 pro-MMP-1 

分 类 号：R781.4[医药卫生—口腔医学]