口腔鳞癌癌周淋巴管生成与颈淋巴结微转移  

The relationship between lymphangiogenesis and cervical lymph node micrometastasis in oral squamous cell carcinoma

作　　者：谢志坚[1] 杨晓峰[1] 范俊[2] 张银凯[1] 吴求亮[1] 谷志远[1] 

机构地区：[1]浙江大学医学院附属口腔医院口腔颌面外科,杭州310006 [2]浙江大学医学院附属第一医院传染病研究所

出　　处：《中华口腔医学杂志》2004年第3期221-223,共3页Chinese Journal of Stomatology

摘　　要：目的　探讨口腔鳞癌癌周淋巴管生成与颈淋巴结微转移的关系。方法　对 4 7例口腔鳞癌组织、10例正常口腔黏膜进行酶组织化学染色 ,检测癌周微淋巴管密度 ;对 10例正常淋巴结、4 7例口腔鳞癌患者的 35 5个淋巴结以高分子量细胞角蛋白AE3单抗为抗体的免疫组织化学方法测定。结果　口腔鳞癌癌周淋巴管密度为 (14 0 4± 6 92 )个 / 2 0 0倍视野下 ,显著高于健康对照组 (5 4 8±2 6 2 )个 / 2 0 0倍视野下 (P <0 0 0 1) ;4 7例患者中角蛋白阳性 2 3例 ,阳性率为 4 8 9% (2 3/ 4 7) ;以淋巴微转移阳性、阴性分组 ,阳性组淋巴管密度为 (16 94± 5 4 3)个 / 2 0 0倍视野下 ,阴性组淋巴管密度为 (11 2 6± 5 0 0 )个 / 2 0 0倍视野下 ,阳性组微淋巴管密度显著高于阴性组 (P <0 0 0 1)。结论　癌周淋巴管密度增高在颈淋巴结微转移中起着非常重要的作用。Objective To probe the relationship between lymphangiogenesis and cervical lymph node micrometastasis in oral squamous cell carcinoma. Methods The microlymphatic vessel density was detected with enzyme histo-chemical method in 47 cases of oral squamous cell carcinoma and 10 cases of normal oral mucosa; 355 lymphnodes were detected with immunohistochemically using monoantibody AE3.Results The mean MLVD was 14.04±6.92 in tumor group,or 5.48±2.62 in normal group. The difference was ( P <0.001). The percentage of tumor with expression CK was 48.9%. The mean MLVD was 16.94±5.43 in CK positive group,or 11.26±5.00 in CK negative group,There was difference significant ( P <0.001).Conclusions Lymphangiogenesis plays a key role in cervical lymph node micrometastasis of oral squamous cell carcinoma.

关 键 词：口腔鳞癌 癌周淋巴管生成 颈淋巴结微转移 检测 淋巴管密度 

分 类 号：R739.8[医药卫生—肿瘤]