IL-2淋巴间隙给药对宫颈癌荷瘤机体特异性T细胞免疫功能的影响  

The T Mediated Immunofunction of Cancer-bearing Mice Treatment of IL-2 Though the Space Perilymphamatically

作　　者：陈杰[1] 曹泽毅[1] 张平[2] 雷巍[1] 毕建宏[2] 潘晓玲[1] 

机构地区：[1]四川大学华西附二院,四川成都610041 [2]四川大学华西基础与法医学院免疫教研室

出　　处：《华西医学》2004年第2期216-218,共3页West China Medical Journal

摘　　要：目的 :通过宫颈癌小鼠爪垫注射IL - 2及 5 -Fu后检测T细胞功能 ,探讨淋巴间隙给药在肿瘤细胞因子生物治疗中的效应。方法 :传代培养的小鼠宫颈癌细胞 (U14 )接种于Balb/c小鼠的左腋部皮下 ,构建荷瘤小鼠动物模型。荷瘤小鼠随机分为IL - 2组、 5 -Fu、IL - 2 +5 -Fu组及生理盐水对照。治疗 10天后分离小鼠脾脏细胞检测CTL细胞杀伤活性、淋巴细胞转化及IL - 2产生情况。结果 :CTL活性分别为 :对照组 :17 6 3±3 75 % ;IL - 2组 :2 7 34± 6 15 % ;5 -Fu组 :19 6 2± 5 17% ;IL - 2 +5 -Fu组 :2 4 0 1± 6 2 3%。淋巴细胞转化指数分别为 :对照组 :0 94± 0 13;IL - 2组 :1 92± 0 2 1;5 -Fu组 :1 30± 0 18;IL - 2 +5Fu组 :1 78± 0 2 8;IL- 2活性分别为 :对照组 :8± 2 1;IL - 2组 :12± 2 2 ;5 -Fu组 :9± 2 5 ;5 -Fu +IL - 2组 :13± 3 2。结论 :宫颈荷瘤小鼠爪垫注射细胞因子能显著性提高荷瘤机体的特异性细胞免疫功能。Objective:Assay the activity of T cell of cervix cancer-bearing mice who therapy by IL-2 or 5-Fu through the space perilymphamatically in this way to investigate the effection of IL-2 in the treatment of cancer through injecting them in the space perilymphamatically. Method: Constructed a cervix cancer-bearing BALB/C mice by transplanted U14 cell line. The cancer-bearing mice were divided into four groups. Treated by IL-2, 5-Fu,IL-2+5Fu or NS separately. Assay the NK and LAK activity of their spleen cells after 10 days of treatment. Result:The activations of CTL are: NS:17.63±3.75%; IL-2:27.34±6.15%; 5-Fu:19.62±5.17%;IL-2+5-Fu:24.01±6.23%.The lymphocyte transform indexes are, NS:0.94±0.13;IL-2:1.92±0.21;5-Fu:1.30±0.18;IL-2+5Fu:1.78±0.28;The activation of IL-2 are:NS:8±2.1;IL-2:12±2.2;5-Fu:9±2.5;5-Fu+IL-2:13±3.2.Conclusion:It can promote the function of T cell mediated immunofunction of cervix cancer bearing mice by injected IL-2 cytokine in the space perilymphamatically.

关 键 词：IL-2 淋巴间隙给药 宫颈癌 荷瘤小鼠 特异性T细胞 免疫功能 生物治疗 

分 类 号：R737.33[医药卫生—肿瘤] R730.54[医药卫生—临床医学]