实验性自身免疫性葡萄膜视网膜炎大鼠T细胞受体V_β8.3基因的表达  

Expression of T cell receptor V_β8.3 gene in rats with experimental autoimmune uveoretinitis

作　　者：张锐[1] 杨培增[1] 褚利群[1] 周红颜[1] 黄祥坤[1] 俞琼[1] 李兵[1] 邢琳[1] 陈璇[1] 纪淑兴[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《中华眼底病杂志》2004年第3期165-167,共3页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金资助项目 (30 1 71 0 0 2 );广东省自然科学基金资助项目 (0 2 1 81 9)

摘　　要：目的　探讨实验性自身免疫性葡萄膜视网膜炎 (EAU)大鼠 CD4 + T细胞抗原受体 (TCR)Vβ8.3基因的表达。　方法　 L ewis大鼠 18只分为 EAU、Freund完全佐剂及空白对照组。用 Fmoc化学合成法合成光感受器间维生素 A类结合蛋白 (IRBP) R16多肽片段 ,以诱导 EAU动物模型。利用磁吸附细胞分选法 (MACS)分离大鼠脾脏 CD4 + T细胞 ,流式细胞术检测 MACS分选前后 CD4 + T细胞比例 ,监测细胞分选效果。通过荧光定量 -聚合酶链反应法检测大鼠脾脏 CD4 + T细胞的 TCR Vβ8.3基因片段的表达。　结果　 IRBP R16免疫 L ewis大鼠稳定地诱导出 EAU动物模型 ;MACS分选 CD4 + T细胞的纯度较分选前明显增高 (P<0 .0 0 1) ;IRBP R16诱导的 EAU大鼠 CD4 + T细胞受体 Vβ8.3基因的表达显著高于空白对照组 (P<0 .0 5 )。　结论　在 IRBP R16诱导的 EAU中存在着抗原特异性 T细胞受体 Vβ8.3基因的优势利用 ,为 EAU的免疫治疗提供了新的思路。Objective To investigate the expression of T cell receptor (TCR) V β8.3 gene on CD4+ T lymphocytes in the rats with experimental autoimmune uveoretinitis (EAU). Methods Eighteen Lewis rats were divided into EAU, complete Freund′s adjuvant, and the control group. Interphotoreceptor retinoid-binding protein (IRBP) R16 peptide was synthesized using Fmoc procedure for induction of EAU. Magnetic absorption cell sorting (MACS) method was used to isolate the CD4+ T lymphocytes from the spleen of the rats. Flow cytometry was used to monitor the efficiency of isolation. The expression of TCR V β8.3 gene segment on CD4+T lymphocytes was determined by fluorescent quantitative polymerase chain reaction. Results EAU was successfully induced in the Lewis rats immunized with IRBP R16 peptide. The proportion of CD4+ T lymphocytes isolated by means of MACS was statistically higher than that before isolation (P<0.001). The expression of TCR V β8.3 gene segment on CD4+ T lymphocytes in EAU rats was significantly higher than that in the control (P<0 05). Conclusions There is a predominant usage of antigen-specific TCR V β 8.3 gene in EAU rats induced by IRBP R16 peptide, which may serve as a target for immunotherapy of EAU.

关 键 词：实验 自身免疫性葡萄膜视网膜炎 大鼠 T细胞受体 Vβ8.3基因 基因表达 

分 类 号：R774.1[医药卫生—眼科]