Percoll fractionation of adult mouse spermatogonia improvesgerm cell transplantation  

作　　者：Kyu-Bom Koh Masatoshi Komiyama Yoshiro Toyama Tetsuya Adachi Chisato Mori 

机构地区：[1]Department of Bioenvironmental Medicine, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan [2]Center for Environment Health and Field Sciences, Chiba University, Kashiwa 277-0882, Japan [3]不详 [4]Department of Genomic Drug Discovery Sciences Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan [5]Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Kawaguchi 332-0012, Japan

出　　处：《Asian Journal of Andrology》2004年第2期93-98,共6页亚洲男性学杂志(英文版)

摘　　要：Aim: To isolate and transplant germ celis from adult mouse testes for transplantation. Methods: In order to distinguish transplanted celis from endogenous celis of recipients, donor transgenic mice expressing green fluores cent protein (GFP) were used. Germ celis were collected from the donors at 10-12 weeks of age and spermatogonia were concentrated by percoll fractionation and transplanted into recipient seminiferous tubules that had been previ ously treated with busulfan at 5 weeks of age to remove the endogenous spermatogenic celis. Results: Twenty vveeks after the transplantation, a wide spread GFP signal was observed in the recipient seminiferous tubules. The presence of spermatogenesis and spermatozoa was confirmed in sections of 12 out of 14 testes transplanted (86 %). However, when germ celis were transplanted without concentration the success rate was zero (0/9). Conclusion: Germ celis from adult mouse testes can be successfully transplanted into recipient seminiferous tubules if the cell population is rich in spermatogonia and the percoll fractionation is useful in obtaining such a cell population.Aim: To isolate and transplant germ celis from adult mouse testes for transplantation. Methods: In order to distinguish transplanted celis from endogenous celis of recipients, donor transgenic mice expressing green fluores cent protein (GFP) were used. Germ celis were collected from the donors at 10-12 weeks of age and spermatogonia were concentrated by percoll fractionation and transplanted into recipient seminiferous tubules that had been previ ously treated with busulfan at 5 weeks of age to remove the endogenous spermatogenic celis. Results: Twenty vveeks after the transplantation, a wide spread GFP signal was observed in the recipient seminiferous tubules. The presence of spermatogenesis and spermatozoa was confirmed in sections of 12 out of 14 testes transplanted (86 %). However, when germ celis were transplanted without concentration the success rate was zero (0/9). Conclusion: Germ celis from adult mouse testes can be successfully transplanted into recipient seminiferous tubules if the cell population is rich in spermatogonia and the percoll fractionation is useful in obtaining such a cell population.

关 键 词：germ cell transplantation green fluorescent protein percoll fractionation spermatogenic cells MICE 

分 类 号：R-332[医药卫生]