Molecular identity, expression and functional analysis of interleukin-1α and its isoforms in rat testis  

作　　者：T. Sultana K. V. Svechnikov K. Gustafsson A. Wahlgren E. Tham G. Weber O. Soder 

机构地区：[1]Pediatric Endocrinology Unit Department of Woman and Child Health [2]Centre for Molecular Medicine and Tumor Biochemistry, Karolinska Institute and Hospital, S-17176 Stockholm, Sweden [3]Department of Biological and Biomedical Sciences, Faculty of Health Sciences, Aga Khan University, Karachi- 74800, Pakistan [4]Department of Chemistry and Biomedical Sciences Vniversity of Kalmar, SE-391 82 Kalmar, Sweden

出　　处：《Asian Journal of Andrology》2004年第2期149-153,共5页亚洲男性学杂志(英文版)

摘　　要：Interleukin-1α (IL-1α) is a proinflammatory cytokine that has also been found to act as a paracrine mediator involved in the regulation of testicular functions. The present review provides an overview of the role of IL 1α in testicular physiology. Bioactive IL-1α isolated from adult rat testis was found to consist of three distinct immunoreactive protein species with apparent sizes of 45, 24 and 19 kDa. These isoforms showed bioactivity in a thymocyte proliferation and steroidogenesis assays with different biopotencies. The background of the molecular heterogeneity and processing, secretion and regulation of the isoforms of testicular IL-1α are discussed. All three isoforms have been found to be secreted into the testis tubular lumen and interstitial space. We have provided evidence that IL-1α is a paracrine factor that may be of importance in, e.g., the regulation of Leydig cell steroidogenesis. Pathophysiologically, testicular IL-1α may contribute to testicular relapse of acute lymphocytic leukemia in boys.Interleukin-1α (IL-1α) is a proinflammatory cytokine that has also been found to act as a paracrine mediator involved in the regulation of testicular functions. The present review provides an overview of the role of IL 1α in testicular physiology. Bioactive IL-1α isolated from adult rat testis was found to consist of three distinct immunoreactive protein species with apparent sizes of 45, 24 and 19 kDa. These isoforms showed bioactivity in a thymocyte proliferation and steroidogenesis assays with different biopotencies. The background of the molecular heterogeneity and processing, secretion and regulation of the isoforms of testicular IL-1α are discussed. All three isoforms have been found to be secreted into the testis tubular lumen and interstitial space. We have provided evidence that IL-1α is a paracrine factor that may be of importance in, e.g., the regulation of Leydig cell steroidogenesis. Pathophysiologically, testicular IL-1α may contribute to testicular relapse of acute lymphocytic leukemia in boys.

关 键 词：IL-1Α SPERMATOGENESIS testis Sertoli celis Leydig celis calpain 

分 类 号：R392[医药卫生—免疫学]