微生物发酵炮制对红花抗氧化活性的影响  

Effect on antioxidative activities of dried flower of Carthamus tinctorius processed by microbes fermentation

作　　者：冯志华[1] 孙启玲[1] 米坤[1] 罗强[2] 

机构地区：[1]四川大学生命科学学院,四川成都610064 [2]乐山师范学院环境与生命科学系,四川乐山614004

出　　处：《中草药》2004年第6期630-633,共4页Chinese Traditional and Herbal Drugs

基　　金：四川省中医药管理局基金资助项目 ( 2 0 0 2 14 )

摘　　要：目的　研究地衣芽孢杆菌 C2 - 13发酵炮制对红花抗氧化活性的影响。方法　邻二氮菲 - Fe2 + 氧化法测定羟自由基清除率 ,比色法测定红细胞的溶血作用、肝脂质过氧化物含量 ,HPL C法分析发酵产物。结果　红花经 C2 - 13的发酵炮制其抗氧化功效显著提高。 HPL C分析还观察到红花中一些成分发生了改变。结论　红花中一些有效成分可能是经 C2 -Object Effects of Bacillus licheniformis (Weigmann) Chester C2-13 on antioxidative activities of dried flower of Carthamus tinctorius L. (DFCT) were studied. Methods Clearance of hydroxyl radical was determined by 1, 10-phenanthroline-Fe^(2+) oxidative assay. The hemolysis of red blood cells, MDA production in hematocytolysis liver homogenate were measured by colorimetric assay. Products of fermentation were analyzed by HPLC. Results C2-13 can increase greatly antioxidative activities of DFCT. Using HPLC assay, it is observed that the fermentation and processing by the strain can improve the effective components of DFCT. Conclusion Some effective components of DFCT may be biotransformed by C2-13, so that the antioxidative activities are promoted.

关 键 词：红花 地衣芽孢杆菌 发酵 炮制 抗氧化 

分 类 号：R286.02[医药卫生—中药学] R286.75[医药卫生—中医学]