烟碱上调大鼠脑纹状体多巴胺D1受体mRNA表达诱导其行为改变  

Chronic nicotine induces changes in locomotor activities of rats by increasing the mRNA levels of dopamine D_1 receptor in striatum

作　　者：陈涛[1] 唐北沙[1] 严新翔[1] 张玉虎[1] 江泓[1] 

机构地区：[1]中南大学湘雅医院神经内科,湖南长沙410008

出　　处：《中国神经科学杂志》2004年第3期213-216,共4页

基　　金：湖南省科委基金 ( 10 13 1)

摘　　要：目的　 通过观察烟碱对大鼠脑纹状体D1,D2 受体mRNA表达的影响 ,研究烟碱诱导大鼠行为改变的可能机制 ,以进一步探讨烟碱对帕金森病具有保护效应的作用机理。 方法 SD大鼠 2 4只 ,随机分为生理盐水组(12只 )、烟碱组 (12只 )。分别给予生理盐水、烟碱 4mg/kg·d,2次 /d ,共 14d。每次注射药物后 0 .5h观察大鼠行为活动 ,并于末次注射药物后 0 .5h处死动物 ,快速分离纹状体 ,采用RT PCR方法检测大鼠纹状体D1,D2 受体mRNA的表达。结果 烟碱组大鼠于用药后第 3天 ,出现走动增多 ,易激惹 ,定型活动明显 ,并于第 714天达到高峰。在大鼠纹状体内 ,烟碱组D1受体mRNA表达比对照组上升 2 3% (分别为 98.6 3± 1.13,6 5 .2 9± 1.4 5 ,P <0 .0 1) ,两组D2 受体mRNA的表达无显著性差异 (P >0 .0 1)。 结论 烟碱可能通过上调大鼠纹状体D1受体mRNA的表达而诱导大鼠理毛、张口等行为改变 ;烟碱可能有部分D1受体激动样作用。ObjectiveTo study the effects of nicotine on gene expression levels of dopamine D 1 and D 2 receptors in striatum and the possible mechanism of nicotine inducing behavioral changes in rat. MethodsTwenty-four Spragne-Dawley rats aged 10 weeks were selected to receive either chronic nicotine administration (4 mg/kg·d , s.c. ) or saline and their locomotor activities were measured 30 min post each injection for 14 d. Then reverse transcription polymerase chain reaction (RT-PCR) was performed to determine the gene mRNA expression levels of dopamine D 1 and D 2 receptors. ResultsWe found locomotor activities of rats increased after nicotine administration for 3 d and reached the top during 7-14 d. The mRNA levels of dopamine D 1 receptor in stiatum increased, while mRNA levels of dopamine D 2 receptor did not change compared with the control rats. ConclusionNicotine may induce changes in locomotor activities by a possible dopaminergic mechanism. It may have partial effects of dopamine D 1 receptor agonist.

关 键 词：烟碱 脑纹状体 多巴胺D1受体 MRNA 表达 诱导 帕金森病 

分 类 号：R742.5[医药卫生—神经病学与精神病学]