苎麻脱胶过程中木聚糖酶最佳作用条件探讨  

Preliminary Study of the Optimum Working Conditions on Xylanse During Process of Ramie Degumming

作　　者：肖丽[1,2] 王贵学[1,2] 陈国娟[1,2] 

机构地区：[1]重庆大学生物工程学院,重庆400030 [2]重庆大学应用生物技术研究中心,重庆400030

出　　处：《重庆大学学报（自然科学版）》2004年第6期48-50,共3页Journal of Chongqing University

基　　金：重庆市科委应用基础研究基金资助项目(7975);重庆江津市锦丰麻业有限公司资助项目

摘　　要：采用均匀试验设计方法,讨论了木聚糖酶在苎麻脱胶过程中,温度、pH值和酶浓度对其酶活力的影响。通过回归分析,得到回归方程和最适酶作用条件:当pH=9.0,酶液=51mL(即生苎麻/g∶酶液/mL=1∶17),温度=50℃时,Y即残胶率是17.629%。经过微量稀碱处理后,残胶率下降到2%左右,达到纺织工业的要求。Effects of temperature, pH and concentration of the xylanase on the enzyme activity of the strain of B-11-3 by with the uniform experimental design. Through stepwise regreesion, a regression equation and the optimum working conditions of the enzyme are obtained pH is 9.0; the volume of the enzyme is 51 mL (ramie/g∶enzyme/mL=1∶17); the temperature is 50 ℃; and the residual gum rate of the ramie fibers after degumming is 17.63%. The residual gum rate of the ramie fibers after degumming with low concentration NaOH solution is over 2%, which is be up to the require of spinning industry.

关 键 词：木聚糖酶 苎麻 脱胶 

分 类 号：Q556[生物学—生物化学]