大鼠血管性痴呆模型海马CA1区神经元MAP-2和NF表达的研究(英文)  

Expression of MAP-2 and NF in CA1 region of hippocampal neurons in rat vascular dementia model

作　　者：刘正清[1] 马志健[2] 李明波[1] 蔡维君[1] 王春旭[1] 文晓丹[1] 

机构地区：[1]中南大学湘雅医学院解剖学和神经生物学系,湖南长沙410013 [2]海南医学院人体解剖学教研室,海南海口570000

出　　处：《中国现代医学杂志》2004年第11期26-30,34,共6页China Journal of Modern Medicine

基　　金：SupportedbyHunanProvinceNaturalScienceFoundation(02JJY2048)

摘　　要：目的观察血管性痴呆大鼠海马CA1区神经元MAP-2、NF表达变化,探讨缺血再灌注损伤后神经元骨架蛋白表达变化与学习记忆的相互关系。方法采用Morris水迷宫筛选空间学习记忆能力正常的雄性Wistar大鼠60只;正常组(Normal);椎动脉焊扎组(VO);双侧颈总动脉结扎20min再灌组(BCCO/R);全脑缺血20min再灌组(GI/R);后三组又分为存活7,14和30天组,处死前作水迷宫检测。用免疫组织化学方法检测MAP-2和NF表达,常规尼氏染色法镜下计数海马CA1区存活神经元。结果水迷宫检测GI/R组大鼠潜伏期增加,尼氏染色证实GI/R组锥体神经元减少(P<0.01)。与Normal组比较,BCCO/R7d组海马CA1区辐射层MAP-2表达减弱,神经元突起中NF表达减少(P<0.05);而BCCO/R14d和30d组胞浆MAP-2和NF表达均无变化(P>0.05)。GI/R组海马CA1区MAP-2、NF的表达在突起中几乎消失,而在锥体神经元核周有强表达,与各对照组比较判别有统计学意义(P<0.05)。结论MAP-2、NF在神经元突起中减少,而在胞体中聚集,是神经元可塑性的体现,在血管性痴呆动物模型中,可能对神经元有保护作用。Objective:To investigate the expression of MAP-2and NF in CA1region of hippocampal neurons and its relation with spatial learning and memory in vascular dementia model(VD).Methods:60Wistar rats with normal learning and memory confirmed by Morris water maze were divided into4groups randomly:①Normal control group;②Bilateral vertebral artery occlusion group(VO);③Bilateral common carotid artery occlusion20min reperfusion group(BCCO/R);④Global cerebral ischemia20min reperfusion group(GI/R).According to animal surviving period,the last three groups were subdivided into three groups,7,14and30days.These rats were tested again in Morris water maze before being sacrificed.Expression of MAP-2and NF were detected by immunohistochemistry and the intensity of immunostaining was measured by MedHPIAS pathologic imaging-analysis system.Coronal slices with Nissl's staining were used to count neuronsin hippocampus CA1regions.Results:Compared with groups normal,VO and BCCO/R,escape latency of GI/R group was longer following elongation of reperfusion(P<0.01).In Hippocampus CA1regions the number of neurons was not reduced in groups VO and BCCO/R,but decreased in GI/R groups(P<0.01),and had signif-icant differences among the GI/R7,14and30d groups(P<0.01).Compared with normal group,expressions of MAP-2and NF in Hippocampus CA1region obviously reduced in BCCO/R7d group(P<0.05),but in groups BCCO/R14d and30d their expressions had no significant change(P>0.05).In groups GI/R the neurites with immunostaining of MAP-2and NF were not observed,but strong staining of these two proteins was present in perikaryon of the neurons(P<0.05).Conclusions:The expression pattern of MAP-2and NF indicates that neuronal plasticity is the responding to injury.The finding that MAP-2and NF were aggregated in cytoplasm might imply their protective roles to neuron in vascular dementia.

关 键 词：血管性痴呆 MORRIS水迷宫 微管相关蛋白-2 神经丝蛋白 

分 类 号：R-332[医药卫生]