百合花发育相关MADS box基因的克隆  

Cloning of Flower Development Associated MADS box Gene Fragments in Lilium

作　　者：张云[1] 刘青林[1] 欧阳青[2] 蔡文启[2] 

机构地区：[1]中国农业大学观赏园艺与园林系,北京100094 [2]中国科学院微生物研究所,北京100080

出　　处：《园艺学报》2004年第3期332-336,共5页Acta Horticulturae Sinica

基　　金：北京市自然科学基金资助项目 ( 60 42 0 15 )

摘　　要：利用RT PCR的方法 ,从百合中克隆得到了 3个接近全长的花发育相关MADSbox基因片段Lf MADS1～ 3。其中LfMADS1和LfMADS3以往未见报道 ,分别与多种作物的AG、SEP同源基因具有较高的同源性 ;LfMADS2与已发表的LMADS2对应区段的氨基酸同源性高达 98 99% 。LfMADS 1-3,three MADS box cDNA fragments associated with flower development were cloned from Lilium using a RT PCR based cloning method. LfMADS 1 and LfMADS 3 are new MADS box genes in lilies,which have not been reported before.They showed high sequence homology with AG and SEP homologs,respectively.The deduced amino acid sequence of LfMADS 2 shared 98 99% identity with the reported LMADS 2, which suggested that they might be the same gene.

关 键 词：百合 花发育 MADS BOX基因 克隆 

分 类 号：S682.2[农业科学—观赏园艺]