植物甜蛋白brazzein基因的克隆与表达  

Cloning and expressing of brazzein gene

作　　者：李广林[1] 郭蔼光[1] 范三红[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北植物学报》2004年第7期1271-1274,共4页Acta Botanica Boreali-Occidentalia Sinica

基　　金：西北农林科技大学重点专题基金资助项目;陕西农业分子生物学实验室重点课题

摘　　要：根据大肠杆菌偏爱的密码子,利用PCR技术体外人工合成brazzeincDNA序列,并将其克隆至原核高效表达载体pET30a(+)中。重组载体pET30a(+)-brazzein转化至大肠杆菌BL21(DE3)中,经IPTG诱导后,SDS-PAGE结果证明pET30a(+)-brazzein在大肠杆菌中获得高效表达,目的蛋白占总菌体蛋白25%左右。Brazzein gene devised by preference condon of E.coli was successfully synthesized through polymerase chain reation (PCR),then it was cloned into prokaryotic expression vector pET30a(+) and expressed in E.coli BL21(DE3) host cells.Upon induction with IPTG,fusion protein was highly produced and the recombinant brazzein was about 25% of total proteins measured with SDS-PAGE.

关 键 词：甜蛋白 BRAZZEIN 基因克隆 基因表达 

分 类 号：Q943.2[生物学—植物学]