常见深部念珠菌感染快速基因诊断  

Rapid gene diagnosis for commom systemic Candida infection

作　　者：李劲松[1] 宋诗铎[1] 祁伟[1] 魏殿军[1] 

机构地区：[1]天津医科大学第二医院感染性疾病研究所,天津300211

出　　处：《中国感染控制杂志》2004年第3期198-201,共4页Chinese Journal of Infection Control

基　　金：天津市科委重点攻关课题资助项目 ( 0 0 3 113 5 11)

摘　　要：目的　研究快速、准确的常见病原念珠菌基因诊断方法 ,为临床深部念珠菌感染的分子诊断奠定基础。方法　收集常见深部念珠菌感染临床株 ,应用多重聚合酶链反应 (PCR)技术扩增念珠菌rDNA的内部转录间隔区ITS1～ITS2 ,根据PCR产物片段的大小进行快速基因鉴定。结果　依据多重PCR电泳图谱将 5 3株常见医院感染念珠菌分为 5型 :(1 )热带念珠菌 1 4株 ,单一条带 ,大小约 35 0bp ;(2 )白色念珠菌 2 5株 ,2条带 ,大小约 1 5 0bp和 5 5 0bp;(3)光滑念珠菌 8株 ,单一条带 ,大小约 90 0bp;(4 )克柔念珠菌 5株 ,单一条带 ,大小约 5 5 0bp ;(5 )乳酒念珠菌 1株 ,单一条带 ,大小约 70 0bp。常见病原菌经PCR扩增均无产物。 结论　快速多重引物PCR诊断结果与常规生化鉴定一致 ,检出率 1 0 0 % ,无假阳性。此方法简单、快速灵敏、特异性高、重复性好 。Objective To study the rapid and accurate method of gene identification for commom pathogenic Candida species and lay the foundation of molecular diagnosis for clinical systemic candidiasis. Methods The clinical strains causing commom systemic Candida infection were collected, a multiple PCR was employed to amplify the internal transcribed spacer (ITS1 ITS2) region of Candida ribosomal DNA. The size of the PCR products was used as the basis of rapid identification. Results Fifty three clinical isolated strains of commom pathogenic Candida were identified as 5 types based on multiplex PCR tests: 14 strains were Candida tropicalis (350 bp), 25 strains Candida albicans (150 bp and 550 bp), 8 strains Candida glabrata (900 bp), 5 strains Candida krusei (550 bp), 1 strain Candida kefyr (700 bp). There were no PCR products to be found for commom pathogenic bacteria. Conclusion Rapid multiple PCR result is fully conform to routine biochemical test, multiple PCR is rapid and convenient method with high specificity and good repetition, and is helpful to early diagnosis for clinical Candida infection.

关 键 词：念珠菌病 基因诊断 实验室技术和方法 

分 类 号：R379.4[医药卫生—病原生物学]