肝炎肝硬化患者外周血Ⅱ型树突状细胞数量和功能变化及意义  

Frequency and function decrease of circulating type 2 pre-dendritic cells(pDC2) and its clinical significance in liver cirrhotic patients with chronic HBV infection

作　　者：段学章[1] 王福生[1] 王敏[2] 庄辉[3] 刘敬超[3] 

机构地区：[1]解放军第三0二医院全军艾滋病与病毒性肝炎防治重点实验室,北京100039 [2]北京大学医学部基础医学院微生物系 [3]北京解放军第三0二医院全军艾滋病与病毒性肝炎防治重点实验室

出　　处：《中华肝脏病杂志》2004年第5期274-277,共4页Chinese Journal of Hepatology

基　　金：国家自然科学基金(30271230)

摘　　要：目的 分析乙型肝炎病毒(HBV)引起的肝炎肝硬化患者外周血Ⅱ型树突状细胞(pDC 2)的数量和产生α干扰素的功能,并分析其与患者淋巴细胞亚群和发生机会性感染的关系。 方法 采用流式细胞分析技术对27例HBV引起的肝炎肝硬化患者进行研究,对患者外周血p DCN和淋巴细胞亚群进行检测;用体外灭活的1型单纯疱疹病毒(HSV—1)刺激并培养外周血单个核细胞(PBMCs),检测培养上清液中。干扰素的产量。 结果 肝炎肝硬化患者pDC2的比例、细胞数和产生α干扰素的功能均降低;pDC2的数量与CD_8^+ T细胞及NK细胞数量高低存在正相关,而且发生机会性感染组患者的pDC2、CD_8^+ T细胞及NK细胞数均低于未感染组。 结论 肝炎肝硬化患者外周血pDC2数量和功能下降,伴随CD_8^+ T细胞和NK细胞数平行降低,与肝炎肝硬化疾病进程和机会性感染有关。Objective To identify the frequency and interferon(IFN)-alpha-producing ability of circulating type 2 pre-dendritic cells(pDC2) and evaluate its role in liver cirrhotic patients with chronic HBV infection. Methods 27 liver cirrhotic patients were included in our study and 25 patients with chronic hepatitis B and 25 healthy individuals were enrolled as controls. The numbers of circulating pDC2 and lymphocytes including CD_4^+ T cells, CD_8^+ T cells, NK cells as well as B cells were analyzed by flow cytometry. The IFN-alpha-producing function of peripheral blood mononuclear cells (PBMCs) representing the circulating pDC2 was determined by ELISA assay after stimulated by ultraviolet-inactivated herpes simplex virus-1(UV-HSV-1). Results The number of pDC2 were(7.21±2.38)×10~6/L, (4.49±3.08)×10~6/L and(2.89±1.17)×10~6/L for healthy control, chronic hepatitis B and cirrhotic patients respectively. Both the number and IFN-alpha-producing function of circulating pDC2 in liver cirrhotic patients significantly lower than that in healthy subjects. There was a correlated simultaneous decrease numbers of circulating CD_8^+ T cells, NK cells in HBV-infected cirrhotic patients. Furthermore, cirrhotic patients with opportunistic infections have lower numbers of pDC2, CD_8^+ T cells and NK cells compared to those without opportunistic infections. Conclusions Liver cirrhotic patients with chronic HBV infection have a significant decrease of circulating pDC2 level and IFN-alpha-producing function. The decreased number and function of pDC2, together with the lower number of CD_8^+ T cells and NK cells may result in the decline of host immune response, which may partially contribute to the disease progression of HBV infection and opportunistic infections.

关 键 词：乙型肝炎 肝硬化 Ⅱ型树突状细胞 Α干扰素 淋巴细胞亚群 机会性感染 流式细胞术 机体免疫 

分 类 号：R575[医药卫生—消化系统] R392.12[医药卫生—内科学]