Monosialoganglioside protected ischemic rat hippocampal slices through stabilizing expression of N-methyl-D-aspartate receptor subunit  

作　　者：Jian-renLIU Mei-pingDING Er-qingWEI Jian-zhengHUANG YingSONG QiangDING Qiu-fuGE 

机构地区：[1]NeurologyDepartment,2ndAffiliatedHospitalofZhejiangUniversitySchoolofMedicine,Hangzhou310009 [2]PharmacologyDepartment,ZhejiangUniversitySchoolofMedicine,Hangzhou310031,China

出　　处：《Acta Pharmacologica Sinica》2004年第6期727-732,共6页中国药理学报（英文版）

摘　　要：AIM: To determine direct protective effect of monosialoganglioside (GM1) on hippocampal slices after oxygen- glucose deprivation and reperfusion (OGD/RP), and investigate the influence on the expression of N-methyl-D- aspartate receptor subunit 1 (NMDAR1) in those hippocampal slices. METHODS: Injury of hippocampal slices and protective effects of GM1 were detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining, toluidine blue staining, and transmission electron microscopy of rat hippocampal slices. Expression of NMDAR1 was detected by Western blot. RESULTS: (1) GM1 at 1.0 μmol/L was the most effective concentration to preserve the TTC staining of the hippocampal slices after OGD/RP (P<0.05), and the next was GM1 at 10.0 μmol/L(P<0.05). (2) Toluidine blue staining and transmission electron microscopy showed GM1 protected the injuried hippocamal slices after OGD/RP. (3) GM1 downregulated the temporally high expression of NMDAR1 in the hippocampal slices immediately after a 25-min OGD and prevented the over low expression of NMDAR1 after a 30-min reperfusion. CONCLUSION: GM1 could protect injuried rat hippocampal slices after OGD/RP through stabilizing the expres- sion of NMDAR1.AIM: To determine direct protective effect of monosialoganglioside (GM1) on hippocampal slices after oxygen- glucose deprivation and reperfusion (OGD/RP), and investigate the influence on the expression of N-methyl-D- aspartate receptor subunit 1 (NMDAR1) in those hippocampal slices. METHODS: Injury of hippocampal slices and protective effects of GM1 were detected by 2,3,5-triphenyltetrazolium chloride (TTC) staining, toluidine blue staining, and transmission electron microscopy of rat hippocampal slices. Expression of NMDAR1 was detected by Western blot. RESULTS: (1) GM1 at 1.0 μmol/L was the most effective concentration to preserve the TTC staining of the hippocampal slices after OGD/RP (P<0.05), and the next was GM1 at 10.0 μmol/L(P<0.05). (2) Toluidine blue staining and transmission electron microscopy showed GM1 protected the injuried hippocamal slices after OGD/RP. (3) GM1 downregulated the temporally high expression of NMDAR1 in the hippocampal slices immediately after a 25-min OGD and prevented the over low expression of NMDAR1 after a 30-min reperfusion. CONCLUSION: GM1 could protect injuried rat hippocampal slices after OGD/RP through stabilizing the expres- sion of NMDAR1.

关 键 词：G(M1) ganglioside brain hypoxia-ischemia hippocampus SPECTROPHOTOMETRY N-methyl-D-aspar-  tate receptors Western blotting 

分 类 号：R743.34[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]