Acetazolamide inhibits aquaporin-1 protein expression and angiogenesis  

作　　者：YangXIANG BingMA TaoLI Jun-weiGAO He-mingYU Xue-junLI 

机构地区：[1]LifeScienceInstitute,NanjingUniversity,Nanjing210093 [2]DepartmentofPharmacology,SchoolofBasicMedicalSciences,PekingUniversity,Beijing100083 [3]NationalResearchInstituteforFamilyPlanning,Beijing100081,China

出　　处：《Acta Pharmacologica Sinica》2004年第6期812-816,共5页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundationof China; No39370286; 39770775;and 30171090.

摘　　要：AIM: To study effects of acetazolamide on aquaporin-1 (AQP1) protein expression and angiogenesis. METHODS: Establishing Lewis-lung-carcinoma model, the localization of AQP1 in tumor tissues was investigated by immuno- histochemical methods; The biological activity of acetazolamide was detected by endothelial cells proliferation test (MTT) assay and chorioallantoic membrane (CAM) vascular inhibition test. RESULTS: Immunohistochemical localization of AQP1in mice tumor was labeled in capillaries, post capillary venules endothelial cells. After being treated with acetazolamide, the number of capillaries and post capillary venules was significantly decreased in tumor tissue. Acetazolamide showed significant inhibitory effect on angiogenesis in CAMand endothelial cell proliferation. CONCLUSION: Acetazolamide might be identified and developed as one of potential lead compounds for a new therapeutic intervention in inhibiting cancer angiogenesis.AIM: To study effects of acetazolamide on aquaporin-1 (AQP1) protein expression and angiogenesis. METHODS: Establishing Lewis-lung-carcinoma model, the localization of AQP1 in tumor tissues was investigated by immuno- histochemical methods; The biological activity of acetazolamide was detected by endothelial cells proliferation test (MTT) assay and chorioallantoic membrane (CAM) vascular inhibition test. RESULTS: Immunohistochemical localization of AQP1in mice tumor was labeled in capillaries, post capillary venules endothelial cells. After being treated with acetazolamide, the number of capillaries and post capillary venules was significantly decreased in tumor tissue. Acetazolamide showed significant inhibitory effect on angiogenesis in CAMand endothelial cell proliferation. CONCLUSION: Acetazolamide might be identified and developed as one of potential lead compounds for a new therapeutic intervention in inhibiting cancer angiogenesis.

关 键 词：NEOPLASMS ACETAZOLAMIDE angiogenesis inhibitors CHIP28 

分 类 号：R318[医药卫生—生物医学工程]