胰岛素样生长因子1及其结合蛋白3在原发性支气管肺癌患者血清中的表达及临床意义  

Significance and Expression of Insulin-like Growth Factor 1 and IGF Binding Protein 3 in Serum of Patients with Lung Cancer

作　　者：王虹[1] 万毅新[1] 张启昆[1] 

机构地区：[1]兰州医学院第二附属医院呼吸内科,甘肃兰州730030

出　　处：《癌症》2004年第6期710-714,共5页Chinese Journal of Cancer

摘　　要：背景与目的:胰岛素样生长因子(insulin-likegrowthfactors,IGFs)对于许多肿瘤细胞系的增殖有强刺激作用,包括肺癌细胞。为探讨IGFs在肺癌发展中的作用,我们对比肺癌患者与对照者血清中IGF1和胰岛素样生长因子结合蛋白3(insulinlikegrowthfactorbindingprotein3,IGFBP3)的表达水平,并研究其与肺癌临床病理参数及生物学行为之间的关系。方法:运用放射免疫法(radioimmunoassay,RIA)检测78例原发性支气管肺癌患者(A组)、39例肺部良性疾病患者(B组)及14例健康人(C组)外周血血清中IGF1的含量,并用免疫放射法(immunoradiometricassay,IRMA)检测IGFBP3的含量。结果:A组血清IGF1、IGFBP3的表达水平(570.67±185.80)μg/L、(3133.60±1110.30)μg/L与B组(466.53±142.42)μg/L、(3694.46±1534.93)μg/L和C组(427.66±141.49)μg/L、(4024.67±1373.31)μg/L相比较,IGF1水平明显升高,差异有显著性(P=0.013,P=0.022),IGFBP3的表达无显著性差异(P>0.05)。IGF1/IGFBP3在A组(0.23±0.16)明显高于B组(0.14±7.12E-02)及C组(0.12±8.2E-02)(P<0.05)。B组与C组之间两种因素的表达均无统计学差异(P>0.05)。IGF1在淋巴结转移的患者中(628.79±177.03)μg/L明显高于无转移者(427.31±119.2)μg/L(P<0.01);BACKGROUND &OBJECTIVE: Insulin-like growth factors (IGFs) can str ongly stimulate the proliferation of a variety of cancer cells, including lung c ancer cells. This study was to discuss the possible role of IGFs in lung cancer development. We examined the expression level of IGF1 and IGF-binding protein 3 (IGFBP3) in plasma of the patients with lung cancer and control subjects, and t he relation of its level with clinical characteristics and biological behaviors of lung cancer was elucidated. METHODS: Seventy-eight cases with lung cancer (A group), 35 with benign pulmonary disease (B group), and 14 healthy controls (C group) were included in this study. Serum IGF1 and IGFBP3 levels were measured w ith radioimmunoassay (RIA) and immunoradiometricassay (IRMA), respectively. RESU LTS: Serum IGF1 level and IGF1/IGFBP3 ratio in A group [X±S,(570.67±185.80) 靏/L,(0.14-02)] and those of C group [(427.66±141.19) 靏/ L,(0.12-02)]. But there was no significance difference (P=0.060) in IGFBP 3 levels between A group [(3133.60±1110.30) 靏/L], B group [(3694.46靏/L], and C group [(4024.67靏/L], and there was no significance i n the expression of IGF1 and IGFBP3 beteween B group and C group (P >0.05). For the cases with lung cancer in pre-chemotherapy, the serum IGF1 level was higher in patients with lymphoid node metastasis, and IGFBP3 level of them was signifi cantly lower than those without metastasis(P< 0.05).Serum IGFBP3 levels were low est in the patients with bone metastases [(1933.14±715.21) 靏/L, P< 0.05]. Fou rteen post-chemotherapy patients showed a lower IGF1 [(480.29±117.93)靏/L] th an those in pre-chemotherapy[(661.76±153.54)靏/L](P< 0.05),whereas IGFBP3 lev el was in inverse to IGF1.CONCLUSION: These results suggested that IGF1 and IGFB P3 play important roles in the growth of lung cancer. Serum examination of them may have potential clinical value.

关 键 词：肺肿瘤 胰岛素样生长因子1 胰岛素样生长因子结合蛋白3 放射免疫法 

分 类 号：R734.2[医药卫生—肿瘤]