次声对大鼠学习记忆行为及海马和颞叶皮层Ryanodine受体的影响  

Effects of infrasound on learning and memory and the expression of ryanodine receptor in hippocampi and temporal cortex of rats

作　　者：谭永霞[1] 李玲[1] 陈景藻[1] 

机构地区：[1]第四军医大学西京医院康复理疗科,西安710032

出　　处：《中华物理医学与康复杂志》2004年第5期272-275,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：全军医学科学技术研究"十五"计划指令性课题 (No.0 1L0 71 )

摘　　要：目的　探讨 8Hz次声对SD大鼠空间学习记忆行为的影响及其作用机制。方法　 40只雄性SD大鼠随机分为正常对照组、90dB、10 0dB及 13 0dB组。各试验组分别暴露于 8Hz次声的 90dB、10 0dB或13 0dB次声舱中 ;对照组每日置次声舱 ,但不给予次声作用。 2组均每天 2h。大鼠每日从次声舱中取出后进行Morris水迷宫训练 ,记录其找到隐匿站台的时间 ,第 6天训练结束后立即取大脑组织 ,并进行免疫组织化学染色 ,光学显微镜下分析其海马及颞叶皮层Ryanodine受体 (RyR)含量的变化。结果　 90dB、10 0dB及 13 0dB组大鼠在水迷宫中找到隐匿站台的时间均比对照组延长 (P =0 .0 10 ,0 .0 0 1,0 .0 0 0 )。 90dB、10 0dB及 13 0dB组大鼠海马及颞叶皮层RyR含量均比对照组减少 (均P =0 .0 0 0 )。大鼠在水迷宫中找到隐匿站台的时间与其海马及颞叶皮层RyR含量呈负相关。结论　 90dB、10 0dB及 13 0dB次声可使大鼠空间学习记忆能力降低 。Objective To investigate the effects of 8Hz infrasound on spatial learning ability of rats and its the underlying mechanism. Methods Forty male SD rats were randomly divided into a control group and an experiment group (consisting of the 8Hz, 90dB, 100dB and 130dB subgroups). The experimental subgroups were exposed to 8Hz, 90dB, 100dB or 130dB infrasound for 2 hours daily, respectively, for a total of 6 days. All of the rats were taken out of the infrasonic depot once a day and given Morris maze training. And at last the brain of the rats were harvested and treated with immunohistochemical staining for detection of ryanodine receptor (RyR). The content of RyR in hippocampi and temporal cortex were detected under an optical microscope. Results The rats in the 8Hz, 90dB, 100dB and 130dB subgroups showed prolonged latency in Morris training ( P =0.010, P =0.001 and P = 0.000 ). The hippocampi and temporal cortex of rats in 8Hz, 90dB, 100dB and 130dB subgroups showed less positive neurons of RyR ( P =0.000). There was a negative correlation between the latency period of rats finding the hidden platform and the content of RyR in hippocampus and temporal cortex. Conclusion Infrasound of 8Hz, 90dB, 100dB and 130dB could decrease the spatial learning ability of rats, and this was possibly related to the reduce of RyR in hippocampus and temporal cortex.

关 键 词：次声 大鼠 学习记忆行为 海马 颞叶皮层 RYANODINE受体 中枢神经系统 

分 类 号：R741[医药卫生—神经病学与精神病学]