基因芯片技术在细小病毒H-1诱导胃癌细胞基因表达研究中的应用价值  

The value of cDNA microarray technology in the studies on the changes of gene expression profiles in gastric carcinoma cells induced by parvovirus H-1 infection

作　　者：刘炯[1] 冉志华[1] 冯缨[1] 邹健[1] 萧树东[1] 

机构地区：[1]上海第二医科大学附属仁济医院上海市消化疾病研究所

出　　处：《中华消化杂志》2004年第5期285-288,共4页Chinese Journal of Digestion

基　　金：德国大众基金会(grantI/75 890 );国家教委青年骨干教师项目

摘　　要：目的　探讨基因芯片技术在细小病毒H 1诱导胃癌细胞HGC2 7相关基因表达改变研究中的应用价值。方法　分别收集H 1病毒感染前与感染 4 8h后胃癌细胞HGC2 7,分离并纯化细胞mRNA ,运用基因芯片技术 ,通过荧光染料标记、芯片杂交、洗涤、扫描及数据分析等步骤 ,得到H 1病毒感染前后胃癌细胞HGC2 7中表达改变的相关基因谱。对基因芯片结果中部分表达有改变的基因进行RT PCR、Northernblot的分析鉴定。结果　胃癌细胞HGC2 7感染H 1病毒 4 8h后的基因芯片结果显示 :在被检测的 80 0 0条目的基因中 ,92 0条基因的表达明显改变 ,β raf、creb、p38 γ、rad2 1、sarp1等部分表达有改变基因的RT PCR和Northernblot鉴定结果显示 ,与表达谱芯片的结果基本一致。结论　基因表达谱芯片技术是大规模平行监测多基因表达的一种有效方法 ,可有效地用于细小病毒HObjective To investigate the role of cDNA microarray techniques in detection of the changes of gene expressional profiles in the gastric carcinoma cell line HGC27 induced by parvovirus H 1 infection. Methods The mRNA of gastric carcinoma cell line HGC27 was collected at the time before or 48 h after H 1 virus infection. Using cDNA microarray, we investigated the diversification of gene expression of HGC27 after H 1 virus infection. A part of genes whose expressions were changed in cDNA microarray analysis were further identified by RT PCR and Northern blot. Results The cDNA microarray analysis showed that the expressions of 920 genes were changed among total of 8000 genes, in which 363 genes showed decreased expressions whilst 557 gene expressions were increased. Among them, some of the genes, including β raf, sarp1, creb, p38 γ and rad21, were further confirmed by RT PCR and Northern blot analysis. The results of RT PCR and Northern blot were coincided well with the cDNA microarray results. Conclusions The cDNA microarray is an effective method for the large scale comparison of multiple genes expressions in a single hybridization and for the studies on anti tumor mechanisms of parvovirus H 1.

关 键 词：基因芯片技术 细小病毒H-1 胃癌细胞 基因表达 肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]