高三尖杉酯碱联合低剂量阿糖胞苷治疗慢粒白血病急变  

Homoharringtonine and low-dose arabinsyl cytosine in the management of late chronic-phase chronic myelogenous leukemia

作　　者：陈林[1] 娄世锋[1] 周慷[1] 张萍[1] 

机构地区：[1]重庆医科大学附属第二医院血液科,400010

出　　处：《重庆医学》2004年第6期891-892,共2页Chongqing medicine

摘　　要：目的　研究高三尖杉酯碱 (homoharringtonine,HHT)以及小剂量阿糖胞苷 (arabinsylcytosine,Ara C)联合诱导慢粒急变细胞株K5 6 2凋亡作用及其机制。为两药的临床联合应用提供理论基础。方法　应用光镜、电镜、Annexin V/PI双染实验、流式细胞分析技术及免疫组织化学实验 ,观察HHT联合小剂量Ara C诱导K5 6 2凋亡模式。结果　HHT联合小剂量Ara C可协同诱导K5 6 2凋亡。机制与降低线粒体膜电位 ,下调bcl xl等有关。结论　高三尖杉酯碱联合低剂量阿糖胞苷治疗慢粒白血病急变简便 ,经济 。Objective To investigate the mechanism of HHT and low dose arabinsyl cytosiwe (Ara c) inducing apoptosis of K562 cells.Methods Apoptosis was analyzed by applying light microscopy,electron microscopy,Annexin V/PI,flow cytometry analysis techniques and immunohistochemical techniques.Results After exposure to HHT and low dose Ara c,K562 cells were induced to apoptosis.Mitochondrial membrane potential and Bcl xl were decreased.Conclusion The combination regimen of HHT and Ara c is effective and economical in patient with late chronic-phase chronic myelogenous leukemia(CML).

关 键 词：高三尖杉酯碱 阿糖胞苷 K562 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]