膀胱癌患者尿脱落细胞存活素表达的临床意义  

Clinical significance of survivin expression on exfoliated urothelial cells of patients with TCCB

作　　者：曹贵华[1] 吴小候[1] 张尧[1] 罗春丽[2] 

机构地区：[1]重庆医科大学附属第一医院泌尿外科,400016 [2]重庆医科大学检验系

出　　处：《中华泌尿外科杂志》2004年第6期377-379,共3页Chinese Journal of Urology

摘　　要：目的　探讨膀胱移行细胞癌 (TCCB)患者尿脱落细胞存活素 (survivin)表达的临床意义。　方法　采用巢式RT PCR方法检测 5 6例TCCB、2 8例对照组 (其中 8例膀胱外泌尿系肿瘤患者、10例非肿瘤泌尿系疾病患者及 10例健康者 )尿脱落细胞存活素的表达。　结果　 5 6例TCCB患者中尿脱落细胞存活素阳性表达 5 1例 (91.1% ) ,T1表达率 90 .5 % (19/ 2 1) ,T2～ 491.4 % (32 / 35 ) ;G1表达率90 .6 % (2 9/ 32 ) ,G2 90 .9% (10 / 11) ,G3 92 .3% (12 / 13) ,存活素表达与临床分期和病理分级不相关 (P >均 0 .0 5 )。对照组 2 8例中仅 1例血尿患者存活素阳性表达 (3.6 % )。TCCB组与对照组存活素阳性率比较差异有显著性意义 (P <0 .0 1)。尿脱落细胞存活素表达诊断TCCB的敏感性为 91.1% ,特异性为96 .4 %。　结论　尿脱落细胞存活素检测诊断TCCB敏感性、特异性高 ,无创 ,可作为诊断TCCB的敏感指标。Objective To determine if detection of survivin expression in urine by using nested RT-PCR can provide a novel noninvasive tool for the diagnosis of TCCB. Methods Nested RT-PCR was used to determine the expression of survivin in exfoliated urothelial cells of 56 patients with TCCB and 28 cases of control group(8 patients with urinary cancers out of bladder,10 patients with non-neoplastic urinary diseases and 10 healthy volunteers). Results 51 patients (91.1%) with TCCB showed positive survivin signals in urine.The positive rates of survivin in T_1 ,T_ 2～4 ,G_1,G_2,G_3 were 90.5%(19/21),91.4%(32/35), 90.6 %(29/32),90.9%(10/11),92.3%(12/13), P >0.05.Only 1 of 28 (3.6%) showed positive survivin signal in the control group.The difference of the positive rate of survivin between groups of TCCB and control was significant ( P <0.01).The sensitivity and specificity of survivin in the diagnosis of TCCB were 91.1% and 96.4%. Conclusions survivin might be a potential biomarker for the noninvasive detection of TCCB by detecting exfoliated urothelial cells with nested RT-PCR.

关 键 词：膀胱癌 尿脱落细胞 存活素 TCCB 肿瘤 

分 类 号：R737.14[医药卫生—肿瘤]