实验性肝纤维化大鼠胶原网络构筑的扫描电镜观察  

SCANNING ELECTRON MICROSCOPE OBSERVATION ON CONSTRUCT OF THE COLLAGEN NETWORK IN CARBON TETRACHLORIDEINDUCED HEPATIC FIBROSIS RATS

作　　者：马瑞琼[1] 张书永[1] 张卫光[1] 田珑[1] 

机构地区：[1]北京大学医学部解剖学与组织胚胎学系,北京100083

出　　处：《解剖学报》2004年第3期302-305,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 170 2 44 ) ;北京大学"985"跨学科项目资助

摘　　要：目的　探讨肝纤维化大鼠胶原网络的三维形态构筑的变化。　方法　采用健康雄性Wistar大鼠 ,随机分为对照组和肝纤维化组 ,后者采用 6 0 %的四氯化碳植物油皮下注射 ,造成肝纤维化大鼠模型 ,分别于实验的第10周和 12周末取材 ,作为 10周和 12周肝纤维化组 ,行天狼猩红显色 ,光镜下观察和氢氧化钠浸渍 ,扫描电镜下观察胶原网络的构筑。　结果　扫描电镜下 ,随着肝纤维化程度的加重 ,增生的胶原纤维交织成网 ,分隔包裹正常肝组织 ,形成大小不等圆形或椭圆型肝组织团块 ,近似于“蜂窝状”囊性的假小叶结构 ;相邻假小叶之间的胶原纤维沉积明显 ,并与包裹门管区和血管的纤维鞘相延续为间隔。与对照组相比 ,10周肝纤维化组形成了尚不完整的假小叶囊 ,12周组的小叶囊致密完整。　结论　肝纤维化时胶原纤维超微构筑发生了改变 ,增生的胶原网络主要由粗、细纤维构成。Objective To explore changes of three-dimensional organization of collagen fibers framework in hepatic fibrosis rats. Methods Healthy adult male Wistar rats(n=30) were randomly divided into control group(n=10) and hepatic fibrosis group.Hepatic fibrosis rats were induced by subcutaneous injections of 60% carbon tetrachloride oily solution.Rats were killed at the end of 10 week and 12 week as 10week(n=10) and 12week(n=10) hepatic fibrosis group.Distribution of collagen was detected by sirius red and polarized microscope,and the architecture was observed under the scanning electron microscope in hepatic fibrosis rats. Results Under the scanning electron microscope,with aggravation of hepatic fibrosis,kinds of collagen fibers and interlaced reticulation compartment were observed,some round or olivary !honeycomb'-like frameworks were formed,which disparted and wrapped up normal liver lobule,and the collagen fiber sheaths formed the deposition of collagen fibers surrounded portal areas or vessels.Pseudolobule vesicles formed in 12week hepatic fibrosis rats were more delicate and intact than that of in 10week hepatic fibrosis rats. Conclusion The microframework of the collagen fibers was changed in hepatic fibrosis.This structure was mainly composed of thick and slight hyperplastic collagen fibers.;

关 键 词：肝纤维化 胶原纤维 扫描电镜 大鼠 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学]