低氧条件下低氧诱导因子-1α及p53的表达  

EXPRESSION OF HYPOXIA INDUCIBLE FACTOR-1α AND p53 IN LUNG OF MICE DURING HYPOXIA

作　　者：宋海邦[1] 瓦龙美[1] 

机构地区：[1]首都医科大学组织学与胚胎学教研室,北京100054

出　　处：《解剖学报》2004年第3期311-315,共5页Acta Anatomica Sinica

基　　金：北京市卫生局科研基金资助

摘　　要：目的　研究低氧时小鼠肺组织中低氧诱导因子 1α及凋亡相关基因p5 3蛋白表达的变化 ,探讨两者之间的关系。　方法　实验用雄性昆明小鼠 ,分为低氧组和对照组 ,低氧仓浓度分别为 10 %、7%、5 %。用免疫荧光组织化学技术及激光共聚焦扫描显微镜技术 ,检测小鼠在低氧条件下肺组织中HIF 1α及p5 3蛋白表达的变化。　结果　正常组小鼠肺组织HIF 1α无表达 ,p5 3少量表达 ;低氧组HIF 1α及p5 3蛋白表达均增加 ,并且随低氧时间的延长及低氧强度的增加而增强 ,P <0 0 5 ,HIF 1α及p5 3的表达呈显著正相关 (r=0 97)。　结论　低氧可诱导小鼠肺组织中HIF 1α的表达增强 ,同时肺组织p5 3蛋白的表达上调 ,两者呈显著正相关 ,说明HIF 1α有可能参与肺组织p5 3蛋白表达的调节 ,进而调节凋亡的发生。Objective To explore the expression of hypoxia inducible factor-1α and p53 in lung during hypoxia. Methods The mice were divided into two group (n=36,male,hypoxia group=36,control group=9).The hypoxia group mice were placed in a hypoxia chamber with 10%,7%,or 5% O 2 respectively.Immunofluorescent histochemical staining technique was used.The staining results were observed under the laser confocal scanning microscope. Results HIF-1α proteins didn't express and p53 proteins expressed weakly in control group mice; Compared with the controls,the expression of HIF-1α and p53 increased dramadically.There was significant difference in expression of HIF-1α and p53 between the hypoxia groups and the control group; Positive relationship was found between the expression of HIF-1α and p53. Conclusion The expression of HIF-1α and p53 increased in lung during hypoxia,which indicates that HIF-1α may play an important role in the expression of p53 and the regulation of apoptosis.;

关 键 词：低氧诱导因子-1Α P53 激光共聚焦扫描显微镜 肺组织 小鼠 

分 类 号：R36[医药卫生—病理学]