氟尿嘧啶引起大鼠气管损伤修复过程中干细胞的定位  

OBSERVATION OF RAT TRACHEAL STEM CELLS DURING THE INJURE AND REGENERATION INDUCED BY 5-FU

作　　者：丁强[1] 贾心善[1] 

机构地区：[1]中国医科大学病理学教研室,沈阳110001

出　　处：《解剖学报》2004年第3期328-330,共3页Acta Anatomica Sinica

基　　金：国家自然基金资助项目 ( 3 0 170 40 7)

摘　　要：目的　气管干细胞的原位观察。　方法　使用 5 氟尿嘧啶 (5 FU)造成大鼠离体气管环的严重损伤 ,采用光镜、PCNA免疫组织化学 ,以及Hoechst33342染色观察气管黏膜的修复过程。　结果　 5 FU作用 12h后 ,气管上皮脱落 ,可见少量间隔分布的类似裸核的细胞呈钉状位于基底膜上 ,PCNA染色阴性 (G0 期细胞 ) ,其中少数细胞Hoechst33342染色阴性。去除 5 FU 6h后 ,气管黏膜由扁平上皮覆盖 ;4 8h后 ,气管环恢复假复层纤毛柱状上皮。结论　 5 FU能杀死处于细胞周期的气管上皮细胞 ,对G0 期细胞作用很小 ,残留于基底膜上的裸核样的G0 期细胞中含有干细胞 ,其Hoechst33342染色阴性 ,并具有排出荧光染料的能力 ,正是这些细胞的增殖分化 ,修复了损伤的气管环。Objective To observe the rat tracheal stem cells in situ.Methods Tracheal rings of Wistar rats treated with 5-FU to make an injure model in vitro then observing the process of regeneration in sequence time from 3*!hours to 48*!hours. Use light microscope to detect the development of tracheal epithelium,assay the level of proliferating cell nuclear antigen (PCNA) immunohistochemistry and perform Hoechst33342 staining to search the negative staining cells. Results Twelve hours after 5-FU it could be seen that tracheal mucosa exfoliated with nuke-nucleus like cells nailed just above the basement membrane,6*!hours after removing 5-FU we could see that the tracheal rings were covered with squamous epithelium. It could be seen that PCNA immunohistochemistry negative staining cells located bordering on the positive one. Meanwhile under fluorescent contrast microscope negative cells with no Hoechst dye combinations were among most of the positive cells. 12*!hours after removing 5-FU mucous granular cells and cilia cells could be seen. After 48*!hours the whole tracheal rings were covered with pseudostratified columnar ciliated epithelium.Conclusion 5-FU is a cell-cycle specific antimetabolite cytotoxicant.It can make the cycling cells degeneration and necrosis but has little effect on the G 0 cells. Few G 0 cells which look like nuke nucleus cells randomly on the basement have the ability to efflux the dye Hoechst33342.The tracheal rings are regenerated through these cells differentiation and proliferation.

关 键 词：气管干细胞 HOECHST33342 损伤 修复 大鼠 

分 类 号：R364[医药卫生—病理学] R329.28[医药卫生—基础医学]