云南汉族白族与纳西族人群幽门螺杆菌iceA基因分布特征及其致病性  

Distribution and pathogenicity of Helicobacter pylori iceA gene in Han,Bai and Naxi nationalities in Yunnan.

作　　者：木金花 周曾芬[2] 南琼[2] 张静祎[2] 王健[2] 金利[2] 和泽元 陶中原 张建中[5] 

机构地区：[1]云南丽江地区医院 [2]昆明医学院第一属医院消化内科 [3]云南丽江县医院 [4]云南大理州医院 [5]中国疾病预防控制中心传染病预防控制所

出　　处：《云南医药》2004年第3期178-181,共4页Medicine and Pharmacy of Yunnan

摘　　要：目的　探讨云南白族、纳西族与汉族幽门螺杆菌 (H pylori)菌株iceAl、iceA2基因型分布特征及 3民族的差异 ,评价该基因与上消化道疾病的关系。方法 (1)选取H pylori相关慢性胃炎和消化性溃疡患者的胃粘膜 ,分离培养H pylori菌株 ;(2 )用PCR方法检测云南白族、纳西族与汉族菌株的iceA1、iceA2基因 ;(3)通过组织病理学观察胃粘膜炎症等级和H pylori定植密度等级。结果 (1) 10 9例H pylori菌株iceA1与iceA2基因的检出率分别为 6 7 0 %与 4 1 3%。其中白族菌株 (31例 )、纳西族菌株 (4 5例 )、汉族菌株 (33例 )iceA1与iceA2基因的检出率分别为 87 1%与 6 1 3% ,4 8 9%与 31 1% ,72 7%与 36 4 % (2 )在云南地区H pylori菌iceA1基因与消化道疾病致病性和组织病理学的关系中 ,仅见云南 10 9例H pylori菌株中iceA1基因在轻度、中度、重度胃粘膜炎症等级中的检出率分别为 76 7% (2 3 30 )、 6 4 4 % (2 9 4 5 )与 85 3% (2 9 34) ,各胃粘膜炎症等级组间检出率有显著性差异 (P <0 0 5 ) ,重度组的检出率高于中组 ;在未观察到、轻度、重度H pylori定植密度患者中的检出率分别为 75 0 % (6 8)、 6 1 8% (2 1 34)、 6 5 5 % (19/ 2 9)、 92 1% (35 38) ,不同程度H pylori定植密度组间检出率有显著性差异 (Objective To investigate the distribution of Helicobacter pylori iceA1 and iceA2 strains of Bai,Naxi and Han nationalities in Yunnan,and to assess the relationship between Helicobacter pylori iceA1 and iceA2 genes and the diseases of upper digestive tract.Methods (1)Isolating Helicobacter pylori strains from gastric mucous membranes in patients with H.pylori related chronic gastritis and peptic ulcer;(2)Amplifying iceA1 and iceA2 genes using polymerase chain reaction(PCR);(3)Observing H.pylori density and inflammation degrees using histopathology.Results(1)The incidences of H.plori iceA1 and iceA2 genes were 67.0%(73/109) and 41.3%(45/109).The incidences of iceA1 and iceA2 genes in Bai(31),Naxi(45) and Han(33) were 87.1%(27/31),61.3%(19/31),48.9(22/45),31.1%(14/45),72.7%(24/33) and 36.4%(12/33) respectively.(2)With respect to the relationship between H.pylori iceA gene and pathogenicity of the diseases of upper digestive tract,only the iceA1 genes of 109 H.pylori strains were detected in 76.7%(23/30),64.4%(29/45) and 85.3%(29/34) in gentle,moderate and serious inflammation degrees respectively.There was significant difference between prevalence of inflammation degrees.There were more prevalent in serious inflammation degrees than moderate.75.0%(6/8),61.8%(21/34),65.5%(19/29) and 92.1%(35/38) of iceA1 genes were detected in normal,gentle,moderate and serious H.pylori density.There was significant difference between prevalence of H.pylori density.There was more prevalent in serious H.pylori density than moderate. Conclusion(1)IceA1 and iceA2 genes show multiple characters and differences in several nationalities in Yunnan Helicobacte pylori.(2) IceA1 is associated with the H.pylori pathogenicity.

关 键 词：幽门螺杆菌 iceAl基因 iceA2基因 

分 类 号：R573.1[医药卫生—消化系统]