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作 者:邱学德[1] 刘乔保[1] 孙力莉[1] 宋建新[1] 王田[1] 肖民辉[1] 杨晓华[1]
机构地区:[1]云南省第一人民医院泌尿外科,云南昆明650032
出 处:《云南医药》2004年第3期192-194,共3页Medicine and Pharmacy of Yunnan
摘 要:目的 探讨大黄素对HER - 2高表达的T2 4和低表达的BIU87膀胱肿瘤细胞株的作用 ,初步明确其用于治疗的可能性。方法 用抗HER - 2单克隆抗体作细胞株的免疫细胞化学 ,用MTT法检测大黄素和阿霉素的细胞抑制率 ,并计算出其相应的IC50 。以IC50 作为参考浓度 ,作用于肿瘤细胞株 ,用流式细胞仪测定细胞凋亡率。结果 T2 4为HER - 2高表达 ,BIU87为低表达。大黄素作用后 72h ,BIU87和T2 4的IC50 分别为 34 2 μM和13 6 μM ,阿霉素的IC50 分别为 19 1μM和 2 5 5 μM。两细胞株的平均IC50 浓度作用于BIU87和T2 4 ,大黄素细胞凋亡率分别为 4 4 5 %和 77 3% (P <0 0 1) ,阿霉素分别为 5 6 9%和 5 7 3% ,P >0 0 5。结论 大黄素对HER- 2高表达之细胞株T2Objective To study the effects of emodin on BIU87, HER-2,and T24 of bladder tumor cell line,and to clear the possibility of emodin as a therapeutic drug.Methods The immunocytochemistry was done with anti-HER-2 monoclonal antibody.The rate of cell inhibition by emodin and adriamycin was detected with MTT.The IC 50of two drugs were calculated and used as a reference concentration in apoptosis test.The apoptosis of tumor cell line was tested with flow cytometer.Results The immunocytochemistry suggested that T24 was high expression of HER-2,and BIU87 was low .The IC 50of BIU87 and T24 showed 34.2μ M and 13.6μM after treated with emodin 72 hours respectively.The adriamycin was 19.1μM and 25.5μM,respectively.After BIU87 and T24 were treated with means of the IC 50 of two cell lines ,after 72 hours,the apoptosis of emodin treated cell lines showed 44.5% and 77.3% respectively(P<0.01).The adriamycin showed 56.9% and 57.3% respectively,(P>0.05).Conclusion The effect of emodin on T24 may be better than BIU87.It is suggested that emodin may become a therapeutic drug.
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