Aβ致PC12细胞的损伤机制与多奈哌齐的神经保护作用  

Mechanism of PC12 Cells Injury Induced by Aβ and Neuroprotective Effect of Donepezil

作　　者：李珂[1] 万琪[1] 魏东[1] 俞英欣[1] 万群[1] 李力[1] 

机构地区：[1]第四军医大学西京医院神经内科,710032

出　　处：《中国临床神经科学》2004年第2期155-158,共4页Chinese Journal of Clinical Neurosciences

摘　　要：目的 :研究Aβ2 5 3 5蛋白诱导PC12细胞损伤类型以及多奈哌齐对其的保护作用。方法 :采用细胞培养、MTT、LDH检测、TUNEL和透射电镜技术 ,观察Aβ2 5 3 5蛋白对PC12细胞的损伤类型以及多奈哌齐对其的保护作用。结果 :单纯Aβ 10 μmol·L-1处理细胞后 12h ,即可检测到LDH浓度明显升高 [( 2 85 0± 15 0 )U·L-1] ;2 4h后 ,TUNEL法检测细胞凋亡百分率为 3 2 % ,明显高于多奈哌齐干预组(P <0 0 1) ,细胞增殖活性降低 ;透射电镜观察可见细胞凋亡和变性坏死两种现象并存。给予多奈哌齐保护组细胞 ,凋亡百分率、LDH浓度分别不同程度升高 ,但明显低于单纯Aβ 10 μmol·L-1处理组 (P <0 0 5 ) ;细胞增殖活性也明显高于单纯处理组。结论 :Aβ引起的细胞损伤中凋亡与变性坏死并存。多奈哌齐对Aβ诱导的这两种损伤都有一定的保护作用。Aim:To study the possible mechanism of cell death induced by soluble Aβ 25-35 and the protective effect of Donepezil on them.Methods:By using cell culture,MTT assays,TUNEL,electron microscope,we measured lactate dehydrogenase(LDH) release rate,cell viability,for observing the effect of Donepezil on Aβ induced apoptosis and necrosis in PC12 cells.Results:MTT assays showed that cell viability decreased under Aβ 25-35 treatment.TUNEL showed that after Aβ treatment the apoptotic cell rate was 32%.Electron microscope showed apoptosis and necrosis occurred.While under Donepezil pretreatement,the apoptotic cell rate was 17% (P<0.01) and LDH release rate decreased compared with the former group.Conclusion:Aβ 25-35 induced PC12 cell apoptosis and necrosis while Donepezil could protect PC12 cells against them both.

关 键 词：损伤机制 多奈哌齐 神经保护作用 细胞凋亡 胆碱酯酶抑制剂 Β淀粉样蛋白 老年性痴呆 

分 类 号：R749.16[医药卫生—神经病学与精神病学]