非小细胞肺癌上皮型钙黏蛋白mRNA的表达与分化转移和病理特征的关系  

Study on the correlation between the level of E-cadherin mRNA expression and pathology,differentiation and metastasis of lung cancer

作　　者：陈晓峰[1] 高文[1] 王海峰[1] 丁嘉安[1] 易祥华[1] 史德刚[1] 

机构地区：[1]上海市肺科医院,上海200433

出　　处：《中国肿瘤临床与康复》2004年第3期200-203,共4页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：上海市卫生局科技发展基金资助项目 ( 9942 4)

摘　　要：目的　研究肺癌中上皮型钙黏蛋白 (E cadherin)mRNA表达水平与肺癌病理学分级和临床分期之间的关系。方法　选择 5 4例肺癌标本、4 6例 (癌旁 )正常肺组织和 5例肺部良性结节性病变组织 ,用RT PCR方法检测标本中E cadherinmRNA的表达水平 ,根据手术、病理和影像学结果判断有无转移并确定分期 ,对E cadherinmRNA表达水平与临床变量之间的关系进行分析。结果　E cadherinmRNA在肺癌和 (癌旁 )正常肺组织中表达阳性率分别为 4 6 .3% (2 5 / 5 4 )和 4 5 .7% (2 1/ 4 6 ) ;低分化、晚期、肺门纵隔淋巴结转移的非小细胞肺癌 ,其E cadherinmRNA的表达量显著降低 (P <0 .0 5 )。结论　E cadherin与肺癌的分化、转移及病理分期有关 。Objective To investigate the relationship between the level of E-cad herin mRNA expression and pathological grades and clinical stages of lung cancer s .Methods Fifty-four specimens of lung c ancer,46 of (para-cancer) normal lung tissues,5 of benign nodal lung diseases were collected.RT-PCR was used to measure the lev el of E-cadherin mRNA expression in the specimens.The stages of disease were de termined according to the results of surgery,pathology and image diagnoses.The n analyses of the relations between the level of E-cadherin mRNA expression and the clinical variables were carried out.Results 46.3% (25/54) and 45.7%(21/46) specimens of lu ng cancer and (para-cancer) normal lung tissue were positive for E-cadherin mR NA expression;non-small cell lung cancer with low differentiation,advanc ed stages and nodal metastases showed a significantly lower expression of E-ca d herin mRNA(P<0.05).Conclusions E-cadher in is related to the differentiation,metastases and pathological staging of lung cancers,hence,might affect their prognosis.

关 键 词：肺肿瘤 上皮型钙黏蛋白基因 MRNA表达 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]