p53蛋白表达和肿瘤微血管生成与肝癌发展及预后的关系  

Correlation of expression levels of p53 and MVD with development and prognosis of hepatocellular carcinoma

作　　者：李志伟[1] 鲁广恩[1] 胡安国[1] 扬五计[1] 戴勇[2] 

机构地区：[1]济南铁路中心医院普外科,济南250001 [2]山东大学齐鲁医院普外科,250012

出　　处：《中国肿瘤临床与康复》2004年第3期213-215,共3页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的　探讨p5 3蛋白表达和肿瘤微血管生成与肝细胞癌 (HCC)发展及预后的关系。方法　采用免疫组化方法检测 5 0例肝细胞癌组织中p5 3蛋白的表达 ,并用抗CD34抗体标记癌组织血管内皮细胞 ,计算肿瘤微血管密度 (microvessldensity ,MVD)。分析p5 3蛋白和MVD与HCC临床病理学特征之间的关系。结果　p5 3阳性表达为癌细胞核棕黄色着色 ,CD34染色定位在血管内皮细胞上 ,HCC的p5 3表达及MVD与肿瘤大小、病灶数目、病理分级、门静脉癌栓形成、包膜是否完整显著相关(P <0 .0 5 )。p5 3蛋白表达阳性者MVD值显著高于阴性者 (P <0 .0 5 ) ;p5 3蛋白表达阳性或MVD≥ 14 8的肝癌患者术后生存时间比p5 3蛋白表达阴性或MVD <14 8者短 (P <0 .0 5 )。结论　p5 3与肿瘤血管生成密切相关 ,p5 3蛋白及MVD可作为肝癌预后判断的一个重要指标。Objective To study the relat io nship between the expression of p53 and microvessel density(MVD) and the clinico pathogical characteristics of human hepatocellular carcinoma(HCC). Method The expression of p53 and CD34 was examined by S-P immunohistochemica l technique in 50 cases of human hepatocellular carcinoma.Resu lts p53 was loca ted in the nuclei of tumor cells and CD34 mainly in the cytoplasm of vascular endothelial cells.MVD was significantly higher in p53-positive HCC than i n p53-negative HCC(P<0.05).p53 expression and MVD were closely correlated to tum or size,number,differentiation,cancer emboli in the portal vein and completeness of capsule(P<0.05).They were also correlated with prognosis of HCC.The mean tumor-free survial time of the patients with MVD≥ 148 was 12 months,while that of MVD<148 was 46 months(P<0.05).Follow-up ex amination showed that postoperative surival time was markedly lower in HCC patients with positiv e expression of p53.Conclusions p53 expressi on is singnificantly associated wi th MVD.The expression of p53 and MVD can be the predictors for the prognosis of HCC.

关 键 词：肝肿瘤 蛋白质 P53 肿瘤血管形成 预后 

分 类 号：R735.7[医药卫生—肿瘤] R730.45[医药卫生—临床医学]