液相色谱–串联质谱法测定化妆品中7种小分子肽含量  

Determination of 7 Kinds of Small Molecular Peptides in Cosmetics by HPLC-MS/MS

作　　者：杨铭 徐建明 王晨蕾 李长于 胡丹 廖上富 

机构地区：[1]浙江方圆检测集团股份有限公司,浙江 杭州 [2]欧诗漫生物股份有限公司,浙江 湖州

出　　处：《分析化学进展》2024年第4期229-236,共8页Advances in Analytical Chemistry

摘　　要：建立了液相色谱–串联质谱法测定化妆品中肌肽等7种小分子肽含量的方法。样品采用1%氯化钠水溶液:甲醇:乙酸 = 6:3:1混合溶剂超声提取,液相分离,质谱外标法定量。结果表明,7种小分子肽在各自范围内线性关系良好(相关系数 > 0.996),检出限在0.05~0.5 μg/L之间,在低、中、高三个添加水平级别,加标回收率在82.7%~109.5%之间,RSD在1.1%~6.7%之间。该方法前处理简单高效,专属性强,灵敏度高,准确性好,可用于化妆品中7种小分子肽的筛查和含量测定。A HPLC-MS/MS method was developed for the determination of 7 small molecular peptides in cosmetics. The samples were extracted with 1% NaCl solution: methanol: acetic acid = 6:3:1 mixed solvent by ultrasonic, separated by liquid phase, and quantified by external standard mass spectrometry. The results showed that the linear relationship of the 7 peptides was good (correlation coefficient > 0.996), and the detection limit was between 0.05~0.5 μg/l, the recoveries ranged from 82.7% to 109.5%, and the RSD ranged from 1.1% to 6.7%. The method is simple and efficient with high specificity, high sensitivity and accuracy. It can be used for screening and determination of 7 small molecular peptides in cosmetics.

关 键 词：液相色谱–串联质谱 化妆品 小分子肽 

分 类 号：R28[医药卫生—中药学]