PCDH10基因DNA甲基化在胃癌中的研究  

Research of PCDH10 DNA Methylation in Gastric Cancer

作　　者：鞠亚菲 姜相君[2] 

机构地区：[1]山东省济宁市兖州区中医医院脾胃病科,山东济宁 [2]青岛市市立医院消化内科,山东青岛

出　　处：《临床医学进展》2016年第1期45-49,共5页Advances in Clinical Medicine

基　　金：青岛市科技局基金资助项目,No.2012-1-3-1-(10)。

摘　　要：目的:探讨PCDH10(原钙粘蛋白10)基因DNA发生甲基化后,其表达与胃癌发生的关系。方法:采用甲基化特异性PCR (methy-lation specific PCR)法和实时荧光定量逆转录–多聚合酶链反应(real-time RT-PCR)方法检测100例胃癌组织及正常组织中PCDH10基因启动子区的甲基化情况。结果:胃癌组织中PCDH10基因的甲基化阳性率较正常组织明显升高,差异具有统计学意义。结论:PCDH10的基因DNA甲基化可能与胃癌发生有关。Objective: To analyze and compare methylation status of PCDH10 gene in gastric cancer and nomal tissues. Methods: Methylation-Specific PCR (MSP) and RT-PCR (real-time RT-PCR) were applied to detect DNA methylation features of the PCDH10 gene in 100 smples. Results: The PCDH10 gene methylation in the gastric cancer tissues was significantly higher than that in normal tissues. Con-clusion: PCDH10 DNA methylation is correlated with gastric cancer.

关 键 词：原钙粘蛋白10 甲基化特异性聚合酶链反应 实时荧光定量逆转录–多聚合酶链反应 胃癌 

分 类 号：R73[医药卫生—肿瘤]