临产前后胎盘上bcl-2、Fasl的表达与母血、羊水中ET-1、PGE₂浓度之间的关系  

A Relationship between the Expressions of bcl-2 and Fasl in Placental and the Concentrations of ET-1 and PGE2 in Maternal Blood and Amniotic Fluid on Labor Onset

作　　者：潘国珍[1] 赵汝波 卢大丽[3] 杨艳[1] 张宏伟 张有成[1] 

机构地区：[1]贵州省人民医院妇产科,贵州贵阳 [2]贵州省人民医院门诊部,贵州贵阳 [3]贵州省人民医院统计室,贵州贵阳

出　　处：《临床医学进展》2017年第5期334-338,共5页Advances in Clinical Medicine

摘　　要：目的:探讨临产前后bcl-2、Fasl在胎盘滋养层细胞的蛋白表达变化及其与母血、羊水中内皮素-1 (Endothelin-1, ET-1)、前列腺素E2 (ProstaglandinE2, PGE2)的浓度之间的关系。方法:53例足月妊娠妇女分成两组,其中临产组28例,未临产组25例。用免疫组化(Immunohistochemical, IHC)法测定胎盘滋养层细胞bcl-2、Fasl的蛋白表达,用放射免疫法测定孕妇母血、羊水中ET-1、PGE2的浓度。结果:1) 临产组胎盘滋养层细胞bcl-2、Fasl的蛋白表达明显低于未临产组,两组平均灰度及阳性细胞率比较,差异有显著性(p 2水平均高于未临产组(p 2的变化与bcl-2的蛋白表达呈负相关(p 0.05)。结论:bcl-2、Fasl介导的胎盘滋养层细胞凋亡在启动分娩发动过程中具有重要作用;bcl-2的作用可能受ET-1、PGE2等细胞因子的调控。Objective: To investigate the expressions of bcl-2 and Fasl proteins in placental trophoblastic cells on labor onset and their relationships with the concentrations of Endothelin-1 and Prostaglandin E2 in maternal blood and amniotic fluid. Methods: Fifty-three women on term pregnancy were divided into two groups. One group on the time of labor onset was twenty-eight women. Another group not on the time of labor onset was twenty-five women. Expressions of bcl-2 and Fasl proteins in placental trophoblastic cells were detected by immunohistochemical assay. The concentrations of ET-1 and PGE2 in maternal blood and amnionotic fluid were detected by radioimmune assay. Results: 1) Expressions of bcl-2 and Fasl proteins in the group of labor onset were significantly lower than that in the group of not labor onset. There was a statistically difference in the average gray scale and the ratio of positive cells between the two groups (p 2 in maternal blood and amniotic fluid of labor onset group were higher than that of not labor onset group (p 2 in maternal blood and amniotic fluid, and the expression of bcl-2 proteins had a negative correlation with the concentrations of ET-1 and PGE2 respectively, but there was no correlation between the expression of Fasl proteins and the concentrations of ET-1 and PGE2. Conclusions: It was considered that the apoptosis of placental trophoblastic cells induced by bcl-2 and Fasl may play an important role in the initiation of term labor onset. ET-1 and PGE2 may control the expression of bcl-2.

关 键 词：胎盘 bcl-2 FASL 内皮素-1 前列腺素E₂ 

分 类 号：R73[医药卫生—肿瘤]