蟾毒灵联合顺铂对骨肉瘤143B细胞凋亡的影响  

The Effect of Bufalin Combined with Ciplatin on Apoptosis of Osteosarcoma 143B Cells

作　　者：贾林 张人龙 王嘉军 张伟[2] 

机构地区：[1]湖北民族大学医学部,湖北 恩施 [2]三峡大学医学院,湖北 宜昌

出　　处：《临床医学进展》2020年第11期2674-2683,共10页Advances in Clinical Medicine

摘　　要：目的:探讨蟾毒灵和顺铂对骨肉瘤(Osteosarcoma, OS) 143B细胞增殖和凋亡的影响及其机制。方法:选取OS-143B细胞为实验细胞,分为阴性对照、蟾毒灵、顺铂和蟾毒灵 + 顺铂联合组四组。MTT法检测细胞增殖;荧光显微镜观察细胞凋亡形态;流式细胞仪检测细胞周期和细胞凋亡;Western blot检测Bcl-2、Bax和Caspase-3蛋白表达。结果:蟾毒灵和顺铂均抑制OS-143B细胞增殖(P 2期阻滞;蟾毒灵与顺铂均可促进OS-143B细胞凋亡,可能与上调Bax,caspase-3蛋白,下调Bcl-2蛋白的表达有关。联合组较单用二药对抑制OS细胞生长、促进其凋亡的作用显著增强。Objective: To investigate the effects and mechanism of bufalin and cisplatin on proliferation and apoptosis of osteosarcoma 143B cells. Methods: OS-143B cells were selected as experimental cells and divided into four groups: the negative control group, the bufalin group, the cisplatin group and the bufalin + cisplatin group. MTT method was used to detect cell proliferation, fluorescence microscope to observe the morphology of apoptosis, flow cytometry to detect cell cycle and apoptosis, and western blot to detect the expression of Bcl-2, Bax and Caspase-3. Results: Both bufalin and cisplatin inhibited the proliferation of OS-143B cells, slowed down the cell growth, blocked S phase and G2 phase, and the apoptosis rate was significantly higher than that in the control group (P

关 键 词：骨肉瘤 蟾毒灵 顺铂 细胞周期 细胞凋亡 

分 类 号：R73[医药卫生—肿瘤]