紫苏醛在烟曲霉菌性角膜炎中的保护作用  

Protective Effect of Perillaldehyde on Aspergillus fumigatus Keratitis

作　　者：贺梦婷 马玉娜 吴媛 马淑芹 赵雪琪 彭旭东[2] 

机构地区：[1]青岛大学,山东 青岛 [2]青岛大学附属医院,山东 青岛

出　　处：《临床医学进展》2022年第2期867-876,共10页Advances in Clinical Medicine

摘　　要：目的:探讨紫苏醛(PAE)对小鼠烟曲霉菌感染性角膜炎的保护作用及机制。方法:我们使用烟曲霉菌感染的C57BL/6小鼠用于体内实验,通过眼前节照相以及炎症评分来评估紫苏醛对于感染小鼠角膜的保护作用。苏木精–伊红(HE)染色用来观察紫苏醛对感染角膜中炎症细胞浸润的影响,过碘酸希夫(PAS)染色观察紫苏醛对感染角膜组织中真菌负荷的影响。采用实时荧光定量PCR (RT-PCR)检测烟曲霉菌感染小鼠角膜及RAW264.7细胞中炎症因子IL-6、MIP-2、CXCL-1及模式识别受体TLR4 mRNA的表达。RT-PCR检测脂多糖(LPS)刺激的RAW264.7细胞中IL-6、MIP-2、CXCL-1的mRNA 表达水平。结果:烟曲霉菌感染的小鼠角膜经过紫苏醛处理后,溃疡面积减小,混浊程度减轻,临床炎症评分下降。紫苏醛处理可抑制角膜中炎症细胞的浸润,减轻真菌负荷。RT-PCR结果显示,紫苏醛处理可及减少烟曲霉菌感染小鼠角膜和RAW264.7细胞中TLR4、IL-6、MIP-2、CXCL-1的mRNA表达水平。我们使用LPS特异性激动TLR4,结果显示,紫苏醛可以抑制由TLR4介导的下游炎症因子IL-6、MIP-2、CXCL-1的mRNA水平的升高。结论:紫苏醛改善小鼠烟曲霉菌性角膜炎的预后,减少炎症细胞的募集及真菌负荷。紫苏醛通过抑制TLR4介导的信号通路,抑制下游炎症因子的释放。Objective: To explore the protective effect of perillaldehyde (PAE) on Aspergillus fumigates (A. fumigatus) keratitis in mice and the following mechanism. Methods: We used A. fumigatus infected C57BL/6 mice for the experiments in vivo. The protective effect of PAE on infected corneas was evaluated by anterior segment photography and clinical score. Hematoxylin-eosin (HE) staining was used to observe the effect of PAE on the infiltration of inflammatory cells in infected corneas, and the fungal load in mice corneas was observed by Periodic Acid Schiff (PAS) staining. RT-PCR was used to detect the mRNA expression of inflammatory cytokines IL-6, MIP-2, CXCL-1 and pattern recognition receptor TLR4 in infected mice corneas and RAW264.7 cells. We also used RT-PCR to measure the mRNA expression of IL-6, MIP-2 and CXCL-1 in RAW264.7 cells stimulated by lipopolysaccharide (LPS). Results: PAE treatment remarkably decreased the area of ulcer, the degree of turbidity, and the clinical score of the mice corneas infected with A. fumigatus. PAE treatment could inhibit the infiltration of inflammatory cells and reduce the fungal load of the infected corneas. RT-PCR results showed that PAE could reduce the mRNA expression of TLR4, IL-6, MIP-2 and CXCL-1 in A. fumigatus infected corneas and RAW264.7 cells. We used LPS to specifically activate TLR4. The results showed that PAE pre-treatment could inhibit the mRNA levels of the downstream inflammatory factors IL-6, MIP-2 and CXCL-1 mediated by TLR4. Conclusion: PAE could improve the prognosis of A. fumigatus keratitis, reduce the infiltration of inflammatory cells and fungal load, and inhibit the release of inflammatory factors mediated by TLR4.

关 键 词：紫苏醛 烟曲霉菌 角膜炎 巨噬细胞 炎症 

分 类 号：R772.21[医药卫生—眼科]