Kiss-1抑制宫颈癌细胞增殖和侵袭的作用机制  

Mechanism of Kiss-1 Inhibiting Proliferation and Invasion of Cervical Cancer Cells

作　　者：宋欧诣 王丽[1] 江速 周金红 杨步琴 

机构地区：[1]广东省深圳市宝安区人民医院,广东 深圳

出　　处：《临床医学进展》2022年第5期4599-4605,共7页Advances in Clinical Medicine

摘　　要：目的:研究kiss-1基因在宫颈癌SiHa细胞增殖活力和侵袭迁徙能力中发挥的作用机制,以便为宫颈癌的治疗提供新的靶点。方法:选取kiss-1基因过表达后稳定转染SiHa宫颈癌细胞。本实验分为3组。1) 空白组为常规宫颈癌SiHa细胞;2) 空载体组为转染空载体的宫颈癌SiHa细胞;3) 过表达组为转染稳定kiss-1基因的宫颈癌SiHa细胞。Western blot法分析3组kiss-1基因蛋白的表达量差异,CCK-8测出3组细胞增殖活力,Transwell模型测3组细胞侵袭能力和转移能力。结果:过表达组kiss-1基因蛋白表达量最高,过表达组中Ecadherin的表达是明显上调(P 【0.05),相反Vimentin的表达明显下调(P 【0.05)。转染kiss-1基因的过表达组细胞增殖活力明显低于其他两组(P 【0.05),尤其在12小时的穿膜细胞个数计数方面,转染kiss-1基因过表达组的个数显著高于其他两组(P 【0.05)。转染kiss-1基因的过表达组可明显的抑制宫颈癌SiHa细胞的侵袭能力和转移能力。结论:kiss-1基因过表达能显著抑制宫颈癌疾病侵袭转移。Objective: To study the mechanism of Kiss-1 in the proliferation and invasion of cervical cancer SiHa cell, and analyze the relative mechanism to supply effective therapy targets to cervical cancer. Methods: Kiss-1 over expression plasmid was transfected into cervical cancer cell lines of SiHa. There were three groups. 1) SiHa control;2) Treated with empty vector;3) Treated with kiss-1 gene vector. Western blot analysis of three groups kiss-1 gene protein expression, cell proliferation via-bility was measured by CCK-8, and cell invasion ability and metastasis ability in the transwell model. Results: After transfection of pEGFPC1/Kiss-1 into cervical cancer cell lines of SiHa (over-express group), the protein of kiss-1 was highest, the expression of E-cadherin was significantly higher (P <0.05);however, the expression of Vimentin was significantly lower than other groups (P <0.05). Migration ability of the cells in over-expression group was decreased than SiHa control (P <0.05). The migrating cells were obviously decreased than SiHa control at 12 h after transfection of pEGFPC1/Kiss-1 into SiHa cells (P <0.05). Over-express group of Kiss-1 could decrease the invasion ability and metastasis ability of cervical cancer SiHa. Conclusion: Over-express of Kiss-1 gene could decrease the potential of invasion and metastasis of cervical cancer.

关 键 词：KISS-1基因 宫颈癌 侵袭 转移 

分 类 号：R737.33[医药卫生—肿瘤]