探究不同浓度七氟醚对七氟醚缺血后处理大鼠的脑保护作用  

To Explore Brain Protection Effect of Different Concentration of Sevoflurane on Sevoflurane Ischemic Postconditioning in Rats

作　　者：郝畅 骆岳峰 宋恺颖 林冰纯 

机构地区：[1]深圳市前海蛇口自贸区医院,麻醉与围手术期医学科,广东 深圳 [2]深圳市宝安区妇幼保健院,麻醉科,广东 深圳

出　　处：《临床医学进展》2023年第8期13303-13308,共6页Advances in Clinical Medicine

摘　　要：目的:探究不同浓度七氟醚对七氟醚缺血后处理大鼠的脑保护作用。方法:选取SPF级成年雄性Wistar大鼠,动物被随机分成5组。假手术组、对照组、0.5 MAC组、1.0 MAC组、1.5 MAC组,使用改良的Pulsinelli四血管闭塞法建立脑缺血模型,使用Zea-Longa评分标准进行评分,于再灌注24 h对各组大鼠进行神经功能缺损评分,评分完成后将大鼠(每组8只)以10%水合氯醛腹腔注射麻醉,断头取脑,测量梗死区域大小。结果:神经功能缺陷评分结果与假手术组比较,其他各组24 h缺损功能评分增加(P = 0.05),与对照组比较,1.0 MAC和1.5 MAC组24 h神经缺损功能评分减轻(P 0.05)。脑梗死面积测量结果1.0 MAC和1.5 MAC七氟烷的后处理明显减少了梗死面积,1.0 MAC组的梗死面积为31.2%,1.5 MAC组为27.6%,远小于对照组的梗死面积45.2%,0.5 MAC组的梗死面积为41.5%,与对照组相比梗死面积差距不大。结论:七氟醚对其缺血后处理的大鼠有一定的脑保护保用,且其保护程度与七氟醚浓度存在一定相关性。Objective: To investigate the protective effect of sevoflurane at different concentrations on the brain of rats treated with sevoflurane ischemia. Methods: Adult male Wistar rats with SPF grade were se-lected and randomly divided into 5 groups. In sham operation group, control group, 0.5 MAC group, 1.0 MAC group and 1.5 MAC group, the cerebral ischemia model was established using the modified Pulsinelli four-vessel occlusion method, and the scores were performed using the Zea-Longa scoring standard. The neurological impairment scores were performed for each group at 24 h after reperfu-sion. After the score was completed, the rats (8 in each group) were anesthetized by intraperitoneal injection of 10% chloral hydrate, and the brains were removed by decapitation to measure the in-farct area size. Result: Compared with sham operation group, 24 h defective function score in-creased in other groups (P = 0.05);compared with contrast group, 24 h defective function score de-creased in 1.0 MAC and 1.5 MAC groups (P 0.05). The results showed that post-treatment with 1.0 MAC and 1.5 MAC sevoflurane significantly reduced the infarct size. The infarct size of the 1.0 MAC group was 31.2%, and that of the 1.5 MAC group was 27.6%, which was much smaller than that of the control group (45.2%). The infarct size of 0.5 MAC group was 41.5%, which was not much different from that of control group. Conclusion: Sevoflurane can protect the brain of ischemic rats, and the degree of protection is related to the concentration of sevoflurane.

关 键 词：七氟醚 改良的Pulsinelli四血管闭塞法 缺血后处理 大鼠 脑保护 

分 类 号：R74[医药卫生—神经病学与精神病学]