基于生物信息学分析CHDH基因在结肠腺癌中的表达及其与预后的关系  

Analysis of CHDH Gene Expression in COAD and Its Relationship with Prognosis Based on Bioinformatics

作　　者：王倚天 厉运凤 张继东[1] 

机构地区：[1]青岛大学附属医院心血管内科,山东 青岛

出　　处：《临床医学进展》2023年第12期18623-18631,共9页Advances in Clinical Medicine

摘　　要：目的:评估CHDH在结肠腺癌中的表达,及其与结肠腺癌患者临床病理特征、预后的关系。方法:在本研究中,我们通过分析源自TCGA数据库的478例结肠腺癌组织和60例正常组织的转录组数据样本,分析CHDH基因在结肠腺癌及癌旁组织中的表达以及与结肠腺癌患者临床特征、预后的关系,随后使用q-PCR验证CHDH在细胞系中的表达,紧接着我们构建蛋白质互作网络,并使用TIMER数据库分析CHDH在不同肿瘤中的表达。结果:CHDH在结肠腺癌肿瘤组织中的表达显著增加(P < 0.001),并且CHDH的高表达与结肠腺癌的临床特征(病理分期、T分期)显著相关(P < 0.05)。CHDH的低表达提示患者预后不佳(Log rank P = 0.0086)。结论:CHDH的表达与结肠腺癌的进展有关,可能成为改善结肠腺癌预后的潜在靶点,为结肠腺癌的治疗提供了新的理论依据。Objective: To evaluate the expression of CHDH in colorectal adenocarcinoma and its relationship with clinicopathological features and prognosis of colorectal adenocarcinoma patients. Methods: In this study, we analyzed the transcriptome data samples of 478 colorectal adenocarcinoma tissues and 60 normal tissues from the TCGA database to analyze the expression of CHDH gene in colorectal adenocarcinoma and adjacent tissues as well as its relationship with clinical characteristics and prognosis of colorectal adenocarcinoma patients. Then q-PCR was used to verify the expression of CHDH in cell lines. Then we constructed a protein interaction network and analyzed the expression of CHDH in different tumors using the TIMER database. Results: The expression of CHDH in colorec-tal adenocarcinoma was significantly increased (P < 0.001), and the high expression of CHDH was significantly correlated with the clinical features (pathological stage, T stage) of colorectal adeno-carcinoma (P < 0.05). Low expression of CHDH suggested poor prognosis (Log rank P = 0.0086). Con-clusion: The expression of CHDH is related to the progression of colorectal adenocarcinoma, which may be a potential target to improve the prognosis of colorectal adenocarcinoma, providing a new theoretical basis for the treatment of colorectal adenocarcinoma.

关 键 词：胆碱脱氢酶 结肠腺癌 蛋白互作网络 TCGA数据库 

分 类 号：R73[医药卫生—肿瘤]