癫痫所致CD57小鼠脑组织海马区小胶质细胞极化后基因表达的研究  

Gene Expression Research after Microglia Polarization in the Hippocampus of CD57 Mouse with Epilepsy

作　　者：贺苗苗 王宇青 王莉 曹相玫 

机构地区：[1]宁夏医科大学基础医学院病理学系,宁夏 银川 [2]永宁县公安局城关派出所,宁夏 银川 [3]宁夏医科大学第一临床医学院,宁夏 银川 [4]宁夏医科大学公共卫生学院,宁夏 银川

出　　处：《临床医学进展》2024年第9期1142-1146,共5页Advances in Clinical Medicine

摘　　要：目的:研究癫痫对C57小鼠脑组织海马区小胶质细胞机化后基因表达的差异。方法:40只C57小鼠随机分为对照组(CNC组12只)及癫痫组PTZ (CPTZ组(戊四氮pentylenetetrazole,PTZ)组28只),CNC组给予生理盐水,CPTZ组给予戊四氮,隔日腹腔注射,共28 d。诱导癫痫过程中,观察小鼠行为,癫痫发作级别、持续时间及结局,实验结束后取材,进行相应转录组测序分析。样本的MethylationEPIC BeadChip实验及数据分析由欧易生物技术有限公司(中国上海)进行。结果:转录组相关测序显示癫痫发作改变小鼠脑组织海马区小胶质细胞M2型的基因表达,其中表达上调基因245个,表达下调基因88个。结论:癫痫发作改变小鼠海马区小胶质细胞的基因异常表达。Objective: To investigate the effects of epilepsy on the mechanized gene expression of microglia in hippocampus of C57 mouse. Methods: 40 C57 mice were randomly divided into control group (12 in CNC group) and PTZ group (28 in CPTZ group). The CNC group was given normal saline, and the CPTZ group was given pentatetrazole by intraperitoneal injection every other day for a total of 28 days. During the process of inducing epilepsy, the behavior, seizure level, duration and outcome of the mice were observed. After the experiment, the samples were collected and analyzed by transcriptome sequencing. Sample MethylationEPIC BeadChip experiments and data analysis were performed by OYI Biotechnology Co., Ltd. (Shanghai, China). Results: Transcriptome-related sequencing showed that epileptic seizure changed the expression of M2 type of microglia in the hippocampus of mouse brain tissue, including 245 up-regulated genes and 88 down-regulated genes. Conclusion: Epileptic seizure changes the abnormal gene expression of microglia in hippocampus of mouse.

关 键 词：癫痫 小胶质细胞 基因表达 转录组测序 

分 类 号：R74[医药卫生—神经病学与精神病学]