血清中NSE、S100β、NT-proCNP与大鼠脓毒症相关性脑病的相关性分析  

Correlation Analysis of Serum NSE, S100β, NT-proCNP and Rat Sepsis-Associated Encephalopathy

作　　者：郑鑫寅 黄文钰 康海[2] 郭倩 

机构地区：[1]青岛大学医学部第四临床医学院,山东 青岛 [2]青岛大学医学院附属烟台毓璜顶医院急诊科,山东 青岛

出　　处：《临床医学进展》2024年第11期160-169,共10页Advances in Clinical Medicine

摘　　要：目的:探讨神经元特异性烯醇化酶(NSE)、C型利钠肽及其氨基末端前肽(NT-proCNP)和中枢神经特异蛋白(S100β)与脓毒症相关性脑病(SAE)的相关性。方法:SD大鼠共计36只,随机数字表法分为两组(n = 18),即假手术组(Sham组)和脓毒症相关性脑病组(SAE组),每组分别在12 h、24 h和48 h进行神经行为学评分并取材。取大鼠心室血离心取上清液,Elisa法检测血清中肿瘤坏死因子-α (TNF-α)、NSE、NT-proCNP、S100β的水平。HE染色观察两组海马组织形态学变化。结果:与Sham组同时间相比,SAE组在24 h和48 h时行为学评分明显降低(SAE组平均数 ± 标准差vs Sham组平均数 ± 标准差,P P α表达在12 h、24 h和48 h均明显提高(SAE组平均数 ± 标准差vs Sham组平均数 ± 标准差,P P P β的表达在24 h和48 h时明显提高(SAE组平均数 ± 标准差vs Sham组平均数 ± 标准差,P β的表达在24 h和48 h时明显提高(SAE组24 h平均数 ± 标准差vs SAE组48 h平均数 ± 标准差,P β水平的升高与SAE发生具有相关性,动态监测脓毒症患者血清中水平的变化有助于早期发现和诊断SAE。Objective: To explore the correlation between neuron specific enolase (NSE), soluble protein-100β (S100β), amino-terminal pro-C-type natriuretic peptides (NT-proCNP) and sepsis-associated encephalopathy (SAE). Methods: A total of 36 Sprague-Dawley rats were equally distributed into two groups (n = 18), namely the Sham group (undergoing Sham surgery) and the SAE group (with sepsis-associated encephalopathy). At 12 h, 24 h, and 48 h, each group underwent neurobehavioral scoring and tissue collection. The serum was extracted from rat ventricular blood through centrifugation for subsequent measurement of tumor necrosis factor-α (TNF-α), NSE, NT-proCNP, and S100β levels using Elisa. HE staining was employed to examine the structural alterations within the hippocampal tissues of the two groups. Results: Compared with the Sham group, the SAE group showed a significant

关 键 词：脓毒症相关性脑病 神经元特异性烯醇化酶 C型利钠肽及其氨基末端前肽 中枢神经特异蛋白 神经行为学评分 肿瘤坏死因子-α 

分 类 号：R74[医药卫生—神经病学与精神病学]