微生物代谢组学及其在土壤环境中的研究进展  

A Review of Microbial Metabolomics in Soil Environment Research

作　　者：肖媛媛 李海波[1] 李英华[1] 杨忠新 

机构地区：[1][[1]东北大学资源与土木工程学院,辽宁沈阳

出　　处：《微生物前沿》2017年第4期116-123,共8页Advances in Microbiology

基　　金：国家自然科学基金面上项目资助(51578115,41571455)。

摘　　要：土壤是多介质互作用的复杂环境,具有过程多样性和各向分异性特征,采用变性梯度凝胶电泳(DGGE)或克隆文库等传统指纹图谱技术研究土壤微生物对外界环境、营养条件等变化的响应机理,存在工作量大,检测限低等局限性,采用非靶向代谢组学方法对低分子量代谢物全谱分析,有可能获得土壤环境微生物研究的新思路,从而突破这一束缚。本文综述了代谢组学特别是微生物代谢组学的分析平台、研究方法及土壤环境微生物代谢组学的研究进展,讨论了利用代谢组学手段揭示污水土地处理系统微生物机制的可行性,提出了微生物代谢组学面临的关键问题与挑战。Soil is a complex environment of multi-media interaction, with specific characteristics of process diversity and anisotropy. There are some limitations, such as the large workload and the low de-tection, which the traditional methods such as DGGE or cloning cannot solve when we do research on the response mechanism of soil microbes to environmental and nutritional conditions. Nevertheless, adopting the non-targeted metabolomics method to analyze spectrum of low molecular weight metabolic may give us the new idea of the soil environment microbe research and break the shackles. This paper reviewed metabolomics especially microbial metabolomics research platform, research methods and research progress of soil microbial metabolomics, discussed the feasibility of using the metabolomics means to reveal the microbial mechanism of the wastewater land treatment system, and put forward some significant problems and challenges.

关 键 词：微生物代谢组学 土壤环境 污水土地处理系统 进展 

分 类 号：Q93[生物学—微生物学]