昆虫杆状病毒基因转移与表达系统在海洋动物中的应用进展  

Advances in the Application of Insect Baculovirus-Mediated Gene Transfer and Expression System in Marine Animals

作　　者：毋梦茜 郭华荣[2] 

机构地区：[1]中国海洋大学,海洋生命学院,海洋生物遗传学与育种教育部重点实验室,山东 青岛 [2]中国海洋大学,海洋生物多样性与进化研究所,山东 青岛

出　　处：《海洋科学前沿》2020年第2期31-36,共6页Advances in Marine Sciences

摘　　要：昆虫杆状病毒介导的基因转移与表达系统是基于Tn7转座酶的转座原理而设计的一种基因转移与表达技术,因其安全性能好、重组蛋白表达量高和表达产物可进行翻译后加工等优点,目前已在哺乳动物、鸟类、昆虫与淡水鱼类等动物中得到广泛应用,但是其在海洋动物中的应用还不多。本文对昆虫杆状病毒介导的基因转移与表达技术在海洋脊椎和无脊椎动物中的应用进行了综述与展望。Baculovirus-mediated gene transfer and expression system was designed to effectively deliver the foreign genes into the target cells by the translocation of Tn7 transposon, the packaging of insect cells and infection of the recombinant baculovirus, with the merits of biological safety, high ex-pression level and post-translational modification of the recombinant proteins. Thus, the baculo-virus-mediated gene transfer and expression system has been widely used in the mammalians, birds, insects and freshwater fishes, but rarely in the marine animals. This review has summarized and prospected the applications of the above-mentioned gene transfer and expression technology in the marine vertebrates and invertebrates.

关 键 词：昆虫杆状病毒基因转移与表达系统 海洋脊椎动物 海洋无脊椎动物 

分 类 号：R73[医药卫生—肿瘤]