褐黄孢链霉菌HBJ591纳他霉素高效发酵工艺研究  

High Efficient Fermentation of Natamycin with Streptomyces Gilvosporeus HBJ591

作　　者：宋慧婷[1] 邓锐 江正兵[1,2] 

机构地区：[1]湖北大学工业生物技术湖北省重点实验室,武汉 [2]湖北大学生命科学学院,武汉

出　　处：《生物过程》2012年第2期65-69,共5页Bioprocess

基　　金：教育部新世纪人才计划(NCET-10-0153);国家高技术研究发展计划(863计划)(2012AA020403);湖北省自然科学基金杰出青年基金(2011CDA080)。

摘　　要：本文以褐黄孢链霉菌HBJ591为基础,研究了补糖工艺对纳他霉素发酵的影响,结合发酵培养基的优化,实现了纳他霉素的高产发酵。结果表明,维持发酵罐中还原糖浓度为2.5%,能有效延长纳他霉素的合成时间。通过响应曲面法对褐黄孢链霉菌HBJ591菌株的发酵培养基进行优化后,结合补糖工艺,在16 L发酵罐中进行补料分批发酵,纳他霉素的发酵水平达到15.7 g/L,比原始发酵工艺的效价提高4.8倍。Based on the study of sugar supplement process, high efficient fermentation with Streptomyces gilvosporeus HBJ591 was carried out via the optimization of the fermentation medium. When the reducing sugar concentration in the fermenter was 2.5%, the synthetic course of natamycin was extended effectively. Response surface method was used to optimize the fermention medium of HBJ591, then fed-batch fermentation was carried out in 16 L fermenter, the natamycin fermentation level reached 15.7 g/L, and the highest yield was 4.8 times higher than the original process.

关 键 词：纳他霉素 响应曲面法 补料发酵 

分 类 号：TQ46[化学工程—制药化工]