RBMX在食管鳞状细胞癌中的相关性研究——RBMX对食管鳞状细胞癌细胞增殖和凋亡的影响  

Study on the Correlation of RBMX in Esophageal Squamous Cell Carcinoma—Effects of RBMX on the Proliferation and Apoptosis of Esophageal Squamous Cell Carcinoma

作　　者：刘芳 李贵才[1,2] 丁宗梅 秦凯 陆松华 

机构地区：[1]南通大学,江苏省神经再生教育部重点实验室,江苏 南通 [2]南通大学,神经再生协同创新中心,江苏 南通 [3]南通大学,医学院,江苏 南通 [4]苏北人民医院,病理科,江苏 扬州 [5]南通大学附属海安医院,胸外科,江苏 海安

出　　处：《生物过程》2021年第3期54-65,共12页Bioprocess

摘　　要：RBMX (也称为hnRNP G)是hnRNP家族的成员,最早被鉴定为表观分子量为43 kDa的核蛋白。本研究目的主要是检验RBMX在ESCC中是否是肿瘤抑制因子的假设。对食管鳞状细胞癌组织进行免疫组织化学和蛋白质印迹分析。采用Kaplan-Meier法来分析ESCC患者的生存率。通过构建RBMX的过表达质粒,研究了RBMX在TE1食管鳞状细胞癌细胞系增殖中的作用。通过CCK-8和流式细胞术测定RBMX对增殖和凋亡的调节。免疫组化分析显示RBMX表达与Ki67呈负相关。生存分析显示RBMX表达与总体生存率显著相关(P 【0.001)。RBMX在TE1细胞中的过表达可导致CyclinD1 (细胞周期蛋白D1)、CDK4和p27水平降低和细胞增殖能力的下降。此外,RBMX的过表达可以诱导TE1细胞凋亡。这些发现表明RBMX参与了食管鳞状细胞癌的增殖,并且RBMX表达水平的升高可能表示食管鳞状细胞癌患者的良好预后。RBMX(also known as hnRNP G), is a member of hnRNP family and was first identifiedas a nuclear protein with an apparent molecular weight of 43 kDa. The currentstudy was designed to test the hypothesis that RBMX is a tumor suppressor.Immunohistochemical and western blot analyses were performed in esophagealsquamous cell carcinoma tissues. Survival analyses were performed by using theKaplan-Meier method. The role of RBMX in proliferation was studied inesophageal squamous cell carcinoma cell lines of TE1 by constructing the RBMXoverexpression plasmid. The regulation of RBMX on proliferation and apoptosis wasdetermined by Cell Counting Kit-8 assays and Flow Cytometry.Immunohistochemical analysis showed that RBMX expression was negativelyassociated with Ki67. Survival analysis revealed that RBMX expression wassignificantly associated with overall survival (P

关 键 词：食管鳞状细胞癌 RBMX 细胞增殖 细胞凋亡 

分 类 号：R73[医药卫生—肿瘤]