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作 者:李国树[1,2] 徐成东 王波[1] 李天星 梁晓华[1]
机构地区:[1]楚雄师范学院化学与生命科学系,楚雄 [2]滇中高原生物资源开发与利用研究所,楚雄
出 处:《植物学研究》2014年第3期105-110,共6页Botanical Research
基 金:云南省植物学重点学科;云南省高校科技创新团队支持计划;楚雄师范学院重点学科建设项目(05YJJSXK03)资助。
摘 要:植物组织培养已广泛应用于农业、林业、医药、化工等领域,在组培中存在经费投入多、能源消耗量大、无菌操作需要大量技术人员等现状。本文综述了组培环节中外植体消毒、培养基支撑物替代与循环利用、应用抗菌剂和抑菌剂抑制培养基及培养物污染、采用自然光照和大棚温度来代替组培日光灯光照等方面降低植物组织培养生产成本、节能降耗的的研究进展,并提出进一步降低组培成本、简化生产工艺的发展方向。Plant tissue culture technology has been widely used in agriculture, medicine and chemical indus- try, etc. But there exist large investment, a large amount of energy consumption, need of a large number of technical personnel for aseptic operation, such as the status quo. This paper compre- hensively introduces the measures to reduce the production cost, save energy and reduce consumption during the whole process of plant tissue culture, which include explant disinfection, alternative and recycling of media supports, application of antibacterial agent and bacteriostasis on the inhibition of media and cultures pollution, using natural light and greenhouse temperature instead of fluorescent lamps. Perspectives to further reduce the costs and simplify the production processes are also put forward in this paper.
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